| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4831635 | B | Binding affinity to recombinant His-tagged ERalpha LBD (307 to 554 residue) (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay | Homo sapiens | 23 | ALA4831478 | assay format | Scientific Literature | |
| 2. | ALA4831636 | B | Binding affinity to ERalpha Y537S mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay | Homo sapiens | 16 | ALA4831478 | assay format | Scientific Literature | |
| 3. | ALA4831637 | B | Binding affinity to ERalpha S463P mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay | Homo sapiens | 16 | ALA4831478 | assay format | Scientific Literature | |
| 4. | ALA4831638 | B | Binding affinity to ERalpha D538G mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay | Homo sapiens | 16 | ALA4831478 | assay format | Scientific Literature | |
| 5. | ALA4831639 | F | Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 72 hrs by CellTiter-Glo assay | Homo sapiens | 23 | ALA4831478 | cell-based format | Scientific Literature | |
| 6. | ALA4831640 | F | Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 7 days by CellTiter-Glo assay | Homo sapiens | 23 | ALA4831478 | cell-based format | Scientific Literature | |
| 7. | ALA4831641 | B | Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression incubated for 48 hrs under hormone-deprived medium by Western blot analysis | Homo sapiens | 5 | ALA4831478 | cell-based format | Scientific Literature |
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