#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4831635	B	Binding affinity to recombinant His-tagged ERalpha LBD (307 to 554 residue) (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	23	ALA4831478	assay format	Scientific Literature
2.	ALA4831636	B	Binding affinity to ERalpha Y537S mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16	ALA4831478	assay format	Scientific Literature
3.	ALA4831637	B	Binding affinity to ERalpha S463P mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16	ALA4831478	assay format	Scientific Literature
4.	ALA4831638	B	Binding affinity to ERalpha D538G mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16	ALA4831478	assay format	Scientific Literature
5.	ALA4831639	F	Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 72 hrs by CellTiter-Glo assay	Homo sapiens	23	ALA4831478	cell-based format	Scientific Literature
6.	ALA4831640	F	Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 7 days by CellTiter-Glo assay	Homo sapiens	23	ALA4831478	cell-based format	Scientific Literature
7.	ALA4831643	B	Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression incubated for 48 hrs by Western blot analysis	Homo sapiens	9	ALA4831478	cell-based format	Scientific Literature