#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4838011	Functional	Antitrypanosomal activity against bloodstream form of wild type Trypanosoma brucei brucei S427 trypomastigotes incubated for 48 hrs by resazurin dye based fluorescence assay	Trypanosoma brucei brucei	11	ALA4837204	organism-based format	Scientific Literature
2.	ALA4838012	Functional	Antitrypanosomal activity against drug resistant Trypanosoma brucei B48 incubated for 48 hrs by resazurin dye based fluorescence assay	Trypanosoma brucei brucei	11	ALA4837204	organism-based format	Scientific Literature
3.	ALA4838013	Functional	Resistance factor, ratio of EC50 for antitrypanosomal activity against drug resistant Trypanosoma brucei B48 to EC50 for antitrypanosomal activity against bloodstream form of wild type Trypanosoma brucei brucei S427 trypomastigotes		11	ALA4837204	assay format	Scientific Literature
4.	ALA4838014	Functional	Antitrypanosomal activity against bloodstream form of Trypanosoma brucei brucei AQP1-3 KO trypomastigotes incubated for 48 hrs by resazurin dye based fluorescence assay	Trypanosoma brucei brucei	9	ALA4837204	organism-based format	Scientific Literature
5.	ALA4838015	Functional	Resistance factor, ratio of EC50 for Antitrypanosomal activity against bloodstream form of Trypanosoma brucei brucei AQP1-3 KO trypomastigotes to EC50 for antitrypanosomal activity against bloodstream form of wild type Trypanosoma brucei brucei S427 trypomastigotes		9	ALA4837204	assay format	Scientific Literature
6.	ALA4838016	ADME	Cytotoxicity against HEK293T cells assessed as reduction in cell viability incubated for 24 hrs by resazurin dye based fluorescence assay	Homo sapiens	11	ALA4837204	cell-based format	Scientific Literature
7.	ALA4838017	Toxicity	Selectivity index, ratio of CC50 cytotoxicity against HEK293T cells to EC50 for antitrypanosomal activity against bloodstream form of wild type Trypanosoma brucei brucei S427 trypomastigotes		10	ALA4837204	cell-based format	Scientific Literature
8.	ALA4838018	Binding	Inhibition of recombinant Trypanosoma brucei brucei alternative oxidase using ubiquinol as substrate preincubated for 2 mins followed by substrate addition by UV-Vis spectrophotometry	Trypanosoma brucei brucei	10	ALA4837204	single protein format	Scientific Literature
9.	ALA4838019	ADME	Drug uptake in bloodstream form of wild type Trypanosoma brucei brucei S427 trypomastigotes assessed as increase in fluorescent signal at 0.2 uM measured by fluorimeter	Trypanosoma brucei brucei	2	ALA4837204	assay format	Scientific Literature
10.	ALA4838021	ADME	Drug uptake in bloodstream form of Trypanosoma brucei brucei AQP1-3 KO trypomastigotes at 0.2 to 2 uM measured after 1 hr by fluorimeter	Trypanosoma brucei brucei	1	ALA4837204	assay format	Scientific Literature