| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4812574 | Binding | Inhibition of BACE-1 (unknown origin) at 2 uM using Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp-OH as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by FRET assay relative to control | Homo sapiens | 15 | ALA4811256 | single protein format | Scientific Literature | |
| 2. | ALA4812575 | Binding | Inhibition of BACE-1 (unknown origin) using Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp-OH as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by FRET assay | Homo sapiens | 15 | ALA4811256 | single protein format | Scientific Literature | |
| 3. | ALA4812576 | Functional | Glutathione peroxidase-like activity in pH 7.5 potassium phosphate buffer assessed as catalytic reduction of H2O2 by measuring initial velocity at 25 degreeC using GSH in presence of NADPH by UV absorbance analysis (Rvb = 30.41+/- 2.06 microM/min) | 15 | ALA4811256 | assay format | Scientific Literature | ||
| 4. | ALA4812577 | ADME | Cytotoxicity against rat PC-12 cells at 25 uM incubated for 24 hrs by MTT assay | Rattus norvegicus | 6 | ALA4811256 | cell-based format | Scientific Literature | |
| 5. | ALA4812578 | Functional | Neuroprotective activity against H2O2-induced cell damage in rat PC-12 cells assessed as decrease in cell death at 10 uM pretreated for 12 hrs followed by H2O2 challenge by MTT assay | Rattus norvegicus | 8 | ALA4811256 | cell-based format | Scientific Literature | |
| 6. | ALA4812580 | Functional | Inhibition of amyloid beta(1 to 40) secretion in human HEK293T cells transfected with human APP harboring Swedish double mutant K595N/M596L measured after 24 hrs by ELISA | Homo sapiens | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 7. | ALA4812582 | Functional | Cytoprotective activity against H2O2- induced cell death in rat PC-12 cells assessed as increase in viable cells pretreated for 12 hrs followed by H2O2 challenge by MTT assay | Rattus norvegicus | 2 | ALA4811256 | cell-based format | Scientific Literature | |
| 8. | ALA4812583 | Functional | Cytoprotective activity against H2O2- induced cell death in rat PC-12 cells assessed as increase in viable cells at 10 uM pretreated for 12 hrs followed by H2O2 challenge by MTT assay relative to control | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 9. | ALA4812584 | Functional | Cytoprotective activity against 6-OHDA induced cell death in rat PC-12 cells assessed as increase in viable cells at 10 uM pretreated for 12 hrs followed by 6-OHDA challenge by MTT assay relative to control | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 10. | ALA4812585 | Functional | Cytoprotective activity against 6-OHDA induced cell death in rat PC-12 cells assessed as decrease in LDH release pretreated for 12 hrs followed by 6-OHDA challenge by LDH assay | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 11. | ALA4812586 | Functional | Cytoprotective activity against H2O2- induced cell death in rat PC-12 cells assessed as decrease in LDH release pretreated for 12 hrs followed by H2O2 challenge by LDH assay | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 12. | ALA4812587 | Functional | Protection against 6-OHDA-induced ROS accumulation in rat PC-12 cells assessed as intracellular ROS level by measuring mean fluorescence intensity pretreated for 12 hrs followed by 6-OHDA challenge by DCFH-DA staining based flow cytometry | Rattus norvegicus | 3 | ALA4811256 | cell-based format | Scientific Literature | |
| 13. | ALA4812588 | Functional | Protection against H2O2-induced ROS accumulation in rat PC-12 cells assessed as intracellular ROS level by measuring mean fluorescence intensity pretreated for 12 hrs followed by H2O2 challenge by DCFH-DA staining based flow cytometry | Rattus norvegicus | 3 | ALA4811256 | cell-based format | Scientific Literature | |
| 14. | ALA4812589 | Functional | Protection against H2O2-induced mitochondrial dysfunction in rat PC-12 cells assessed as change in mitochondrial membrane potential at 2.5 uM pretreated with compound for 12 hrs followed by H2O2 challenge by JC-1 staining based flow cytometry (Rvb = 73 %) | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 15. | ALA4812590 | Functional | Protection against H2O2-induced mitochondrial dysfunction in rat PC-12 cells assessed as change in mitochondrial membrane potential at 5 uM pretreated with compound for 12 hrs followed by H2O2 challenge by JC-1 staining based flow cytometry (Rvb = 73 %) | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 16. | ALA4812591 | Functional | Protection against H2O2-induced mitochondrial dysfunction in rat PC-12 cells assessed as change in mitochondrial membrane potential at 10 uM pretreated with compound for 12 hrs followed by H2O2 challenge by JC-1 staining based flow cytometry (Rvb = 73 %) | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 17. | ALA4812592 | Functional | Prevention of 6-OHDA-induced calcium overload in rat PC-12 cells assessed as reduction in intracellular free calcium pretreated with compound for 12 hrs followed by 6-OHDA challenge by Fluo-3AM based fluorescence assay | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 18. | ALA4812593 | Functional | Prevention of H2O2-induced calcium overload in rat PC-12 cells assessed as reduction in intracellular free calcium pretreated with compound for 12 hrs followed by H2O2 challenge by Fluo-3AM based fluorescence assay | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 19. | ALA4812594 | Functional | Protection against 6-OHDA-induced mitochondrial dysfunction in rat PC-12 cells assessed as change in mitochondrial membrane potential at 2.5 uM pretreated with compound for 12 hrs followed by 6-OHDA challenge by JC-1 staining based flow cytometry (Rvb = 56 %) | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature | |
| 20. | ALA4812595 | Functional | Protection against 6-OHDA-induced mitochondrial dysfunction in rat PC-12 cells assessed as change in mitochondrial membrane potential at 5 uM pretreated with compound for 12 hrs followed by 6-OHDA challenge by JC-1 staining based flow cytometry (Rvb = 56 %) | Rattus norvegicus | 1 | ALA4811256 | cell-based format | Scientific Literature |
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