Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter 96 aqueous one solution reagent based assay
Bactericidal activity against Mycobacterium bovis BCG ATCC 35734 assessed as reduction in colony forming count at 4 times MIC measured over 28 days by time-kill assay
Bactericidal activity against Mycobacterium bovis BCG ATCC 35734 assessed as fold reduction in viability at 4 times MIC measured over 14 days by time-kill assay
Ratio of MIC for antimycobacterial activity against Mycobacterium bovis BCG ATCC 35734 in absence of glycerol to MIC for antimycobacterial activity against Mycobacterium bovis BCG ATCC 35734 in presence of glycerol
Bactericidal activity against Mycobacterium bovis BCG ATCC 35734 assessed as fold reduction in viability at 40 times MIC90 preincubated for 20 days under oxygen deprived condition followed by compound addition and measured after 5 days
Bactericidal activity against Mycobacterium tuberculosis H37Rv ATCC 27294 assessed as reduction in colony forming unit at 40 times MIC90 preincubated for 20 days under nutrient deprived condition followed by compound addition and measured after 7 days
Induction of membrane permeabilization in Mycobacterium bovis BCG ATCC 35734 at 4 times MIC after 8 hrs by SYTO9 and PI staining based fluorescence assay
Induction of cell envelope stress in Mycobacterium bovis BCG ATCC 35734 expressing piniBAC RFP at 100 times MIC90 after 24 hrs by fluorescence analysis
Antimycobacterial activity against Mycobacterium bovis BCG ATCC 35734 carrying katG loss-of-function mutant assessed fold reduction in MIC at 1 to 2 times of MIC90 incubated for 5 days
Antimycobacterial activity against Mycobacterium tuberculosis H37Rv ATCC 27294 assessed as low spontaneous mutation frequencies at 1 to 8 times MIC90 incubated for 8 weeks