#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4834203	Binding	Activation of recombinant human PKM2 at 10 uM incubated 30 mins by fluorescence microplate reader analysis	Homo sapiens	2	ALA4831557	single protein format	Scientific Literature
2.	ALA4834204	Functional	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
3.	ALA4834205	Functional	Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
4.	ALA4834206	Functional	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
5.	ALA4834207	Functional	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
6.	ALA4834208	Functional	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
7.	ALA4834209	Functional	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
8.	ALA4834210	Functional	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	38	ALA4831557	cell-based format	Scientific Literature
9.	ALA4834211	ADME	Cytotoxicity against human NCM460 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	Homo sapiens	2	ALA4831557	cell-based format	Scientific Literature
10.	ALA4834212	Toxicity	Selectivity index, ratio of IC50 for human NCM460 cells to IC50 for human HT-29 cells	Homo sapiens	2	ALA4831557	assay format	Scientific Literature
11.	ALA4834213	Unassigned	Selectivity index, ratio of IC50 for human NCM460 cells to IC50 for human SW480 cells	Homo sapiens	2	ALA4831557	assay format	Scientific Literature
12.	ALA4834214	Binding	Activation of recombinant human PKM2 incubated 30 mins by fluorescence microplate reader analysis	Homo sapiens	1	ALA4831557	single protein format	Scientific Literature
13.	ALA4834215	Binding	Binding affinity to recombinant human PKM2 assessed as covalent adduct formation incubated for 1 hr by LC-MS/MS analysis	Homo sapiens	1	ALA4831557	single protein format	Scientific Literature
14.	ALA4834216	Functional	Effect on total PKM2 protein expression in nucleus of human HT-29 cells at 0.45 to 1.8 uM after 48 hrs by Western blot analysis	Homo sapiens	1	ALA4831557	tissue-based format	Scientific Literature
15.	ALA4834217	Functional	Effect on total PKM2 protein expression in cytoplasm of human HT-29 cells at 0.45 to 1.8 uM after 48 hrs by Western blot analysis	Homo sapiens	1	ALA4831557	tissue-based format	Scientific Literature
16.	ALA4834218	Binding	Inhibition of PKM2 dimer nuclear translocation in human HT-29 cells by immunostaining based analysis	Homo sapiens	1	ALA4831557	cell-based format	Scientific Literature
17.	ALA4834219	Binding	Activation of PKM2 in human HT-29 cells assessed as increase in PKM2 tetramer formation at 0.6 to 1.2 uM after 48 hrs by Western blot analysis	Homo sapiens	1	ALA4831557	cell-based format	Scientific Literature
18.	ALA4834220	Binding	Activation of PKM2 in human HT-29 cells assessed as increase in PKM2 tetramer formation by dynamic laser light scattering analysis	Homo sapiens	1	ALA4831557	cell-based format	Scientific Literature
19.	ALA4834221	Binding	Activation of PKM2 in human HT-29 cells assessed as increase in cell basal respiration	Homo sapiens	1	ALA4831557	cell-based format	Scientific Literature
20.	ALA4834222	Binding	Activation of PKM2 in human HT-29 cells assessed as decrease in extracellular acidification rate	Homo sapiens	1	ALA4831557	cell-based format	Scientific Literature