# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4834652 | B | Inhibition of recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells at enzyme concentration 9 nM using Arg-AMC as substrate preincubated for 15 mins followed by substrate addition and measured every 10 mins for 300 mins by fluorescence based assay | Homo sapiens | 27 | cell-based format | Scientific Literature | ||
2. | ALA4834653 | B | Binding affinity to recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells assessed as change in melting temperature at 125 uM incubated for 30 mins using SYPRO orange dye by RT-PCR-based fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
3. | ALA4834654 | B | Binding affinity to recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells assessed as change in melting temperature at 250 uM incubated for 30 mins using SYPRO orange dye by RT-PCR-based fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA4834655 | B | Binding affinity to recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells assessed as change in melting temperature at 500 uM incubated for 30 mins using SYPRO orange dye by RT-PCR-based fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
5. | ALA4834656 | B | Binding affinity to recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells assessed as change in melting temperature at 1000 uM incubated for 30 mins using SYPRO orange dye by RT-PCR-based fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature |