# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4818906 | Binding | Covalent inhibition of recombinant human GST-tagged BTK (2 to 659 end residues) expressed in baculovirus expression system assessed as inactivation constant using NH2-KKKAPFSWYLPEEG as substrate measured every 2 mins for 2 hrs in presence of ATP by microplate reader assay | Homo sapiens | 15 | ALA4814076 | assay format | Scientific Literature | |
2. | ALA4818907 | Binding | Covalent inhibition of recombinant human GST-tagged BTK (2 to 659 end residues) expressed in baculovirus expression system assessed as inhibition constant using NH2-KKKAPFSWYLPEEG as substrate measured every 2 mins for 2 hrs in presence of ATP by microplate reader assay | Homo sapiens | 15 | ALA4814076 | assay format | Scientific Literature | |
3. | ALA4818908 | Binding | Covalent inhibition of recombinant human GST-tagged BTK (2 to 659 end residues) expressed in baculovirus expression system assessed as Kinact/Ki ratio using NH2-KKKAPFSWYLPEEG as substrate measured every 2 mins for 2 hrs in presence of ATP by microplate reader assay | Homo sapiens | 15 | ALA4814076 | assay format | Scientific Literature | |
4. | ALA4818909 | Binding | Inhibition of BTK in C57Bl/6 mouse splenocyte assessed as reduction in anti-IgM-induced CD69 expression incubated for 1 hr followed anti-IgM stimulation and measured after overnight by flow cytometry analysis | Mus musculus | 14 | ALA4814076 | tissue-based format | Scientific Literature | |
5. | ALA4818910 | Binding | Inhibition of BTK in Wistar rat whole blood assessed as reduction in anti-IgD-induced B220/CD86+ expression incubated for 1 hr followed anti-IgD stimulation and measured after overnight by flow cytometry analysis | Rattus norvegicus | 14 | ALA4814076 | tissue-based format | Scientific Literature |