#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5033288	B	Displacement of [3H]-diprenorphine from mouse KOR expressed in HEK293 cell membranes by radioligand binding assay relative to control	Mus musculus	20		cell membrane format	Scientific Literature
2.	ALA5033289	F	Agonist activity at mouse KOR expressed in HEK293 cell assessed as maximal inhibition of cAMP concentration incubated for 1 hrs by time-resolved fluorescence resonance energy transfer assay relative to control	Mus musculus	20		cell-based format	Scientific Literature
3.	ALA5033290	B	Displacement of [3H]-diprenorphine from mouse KOR expressed in HEK293 cell membranes at 10 uM by two-point binding assay	Mus musculus	1		cell membrane format	Scientific Literature
4.	ALA5033291	F	Agonist activity at mouse KOR expressed in HEK293 cell assessed as inhibition of cAMP concentration incubated for 1 hrs by time-resolved fluorescence resonance energy transfer assay	Mus musculus	20		cell-based format	Scientific Literature
5.	ALA5033292	B	Displacement of [3H]-diprenorphine from mouse KOR expressed in HEK293 cell membranes at 100 nM by two-point binding assay	Mus musculus	1		cell membrane format	Scientific Literature
6.	ALA5033293	B	Displacement of [3H]-diprenorphine from mouse MOR expressed in HEK293 cell membranes by radioligand binding assay relative to control	Mus musculus	1		cell membrane format	Scientific Literature
7.	ALA5033294	B	Displacement of [3H]-diprenorphine from mouse DOR expressed in HEK293 cell membranes by radioligand binding assay relative to control	Mus musculus	1		cell membrane format	Scientific Literature
8.	ALA5033295	F	Agonist activity at EGFP-tagged mouse KOR expressed in HEK293 cells co-expressing beta-arrestin2 nano-luciferase assessed as beta-arrestin2 recruitment for 5 mins by BRET assay	Mus musculus	2		cell-based format	Scientific Literature
9.	ALA5033296	F	Agonist activity at EGFP-tagged mouse KOR expressed in HEK293 cells co-expressing beta-arrestin2 nano-luciferase assessed as maximal beta-arrestin2 recruitment for 5 mins by BRET assay relative to control	Mus musculus	1		cell-based format	Scientific Literature
10.	ALA5033297	A	Stability of the compound in simulated gastric fluid at pH 1.2 assessed as drug degradation by UPLC-UV analysis		2		assay format	Scientific Literature
11.	ALA5033298	A	Effect on healthy mouse assessed as visceromotor response measured at different colorectal distention pressure at 10 uM intra-colonic administration as enema by electromyography	Mus musculus	1		organism-based format	Scientific Literature
12.	ALA5033299	F	Reduction in visceromotor response in chronic visceral hypersensitivity-induced chronic abdominal pain mouse model measured at different colorectal distention pressure at 10 uM intra-colonic administration as enema by electromyography	Mus musculus	1		organism-based format	Scientific Literature
13.	ALA5033300	F	Analgesic activity in chronic visceral hypersensitivity-induced chronic abdominal pain mouse model assessed as reduction in mechanical stimulation induced action potential firing of colonic nociception at 1 uM measured at 5 mins by Von Frey filament assay	Mus musculus	1		organism-based format	Scientific Literature
14.	ALA5033301	F	Analgesic activity in chronic visceral hypersensitivity-induced chronic abdominal pain mouse model assessed as reduction in colonic nociceptor mechano-sensitivity at 1 uM in presence of 0.1 uM nor-BNI measured at 5 mins by Von Frey filament assay	Mus musculus	1		organism-based format	Scientific Literature
15.	ALA5033302	T	Toxicity in mouse assessed as motor coordination by measuring latency to fall in mouse at 5 mg/kg, ip measured at 45 mins by rotarod test	Mus musculus	1		organism-based format	Scientific Literature
16.	ALA5033303	T	Toxicity in mouse assessed as motor coordination by measuring vocalization in mouse at 5 mg/kg, ip measured at 60 mins by rotarod test	Mus musculus	1		organism-based format	Scientific Literature
17.	ALA5033304	T	Toxicity in mouse assessed as motor coordination by measuring flinch response in mouse at 5 mg/kg, ip measured at 60 mins by rotarod test	Mus musculus	1		organism-based format	Scientific Literature
18.	ALA5033305	T	Toxicity in mouse assessed as motor coordination by measuring jump response in mouse at 5 mg/kg, ip measured at 60 mins by rotarod test	Mus musculus	1		organism-based format	Scientific Literature