#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5045981	Binding	Protac activity at VHL/HDAC2 in human HCT-116 cells assessed as reduction in LSD1 level measured after 48 hrs by Western blot analysis	Homo sapiens	3	ALA5042553	cell-based format	Scientific Literature
2.	ALA5045984	Binding	Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as induction of HDAC1 degradation at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	5	ALA5042553	cell-based format	Scientific Literature
3.	ALA5045985	Binding	Protac activity at VHL/HDAC2 in human HCT-116 cells assessed as induction of HDAC2 degradation at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	6	ALA5042553	cell-based format	Scientific Literature
4.	ALA5045986	Binding	Protac activity at VHL/HDAC3 in human HCT-116 cells assessed as induction of HDAC3 degradation at 10 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	2	ALA5042553	cell-based format	Scientific Literature
5.	ALA5046016	Binding	Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as reduction in LSD1 level measured after 48 hrs by Western blot analysis	Homo sapiens	3	ALA5042553	cell-based format	Scientific Literature
6.	ALA5046020	Functional	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by cell-titer glo assay	Homo sapiens	5	ALA5042553	cell-based format	Scientific Literature
7.	ALA5046021	Functional	Induction of apoptosis in human HCT-116 cells assessed as downregulation of E2F1 level at 10 uM incubated for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
8.	ALA5046022	Functional	Induction of apoptosis in human HCT-116 cells assessed as downregulation of CDK1 level at 10 uM incubated for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
9.	ALA5046023	Functional	Induction of apoptosis in human HCT-116 cells assessed as downregulation of cyclin El level at 10 uM incubated for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
10.	ALA5046024	Functional	Induction of apoptosis in human HCT-116 cells assessed as upregulation of p21 level at 10 uM incubated for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
11.	ALA5046025	Functional	Induction of apoptosis in human HCT-116 cells assessed as upregulation of p15 level at 10 uM incubated for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
12.	ALA5046026	Functional	Induction of apoptosis in human HCT-116 cells assessed as increase in expression of TP63 at 10 uM preincubated with HDAC inhibitor incubation for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
13.	ALA5046027	Functional	Induction of apoptosis in human HCT-116 cells assessed as increase in expression of PMAIP1 at 10 uM incubation for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
14.	ALA5046028	Functional	Induction of apoptosis in human HCT-116 cells assessed as increase in expression of DHSRS2 at 10 uM incubation for 24 hrs	Homo sapiens	4	ALA5042553	cell-based format	Scientific Literature
15.	ALA5107748	Binding	Inhibition of class I HDAC in human HCT-116 cells nucleus extract assessed as fold change in H3K56 acetylation level incubated for 24 hrs by Western blot analysis relative to control	Homo sapiens	2	ALA5104222	subcellular format	Scientific Literature
16.	ALA5107782	Binding	PROTAC activity at VHL/HDAC1 in human HCT-116 cells assessed as HDAC1 level incubated for 24 hrs by Western blot analysis relative to control	Homo sapiens	2	ALA5104222	cell-based format	Scientific Literature
17.	ALA5107783	Binding	PROTAC activity at VHL/HDAC2 in human HCT-116 cells assessed as HDAC2 level incubated for 24 hrs by Western blot analysis relative to control	Homo sapiens	2	ALA5104222	cell-based format	Scientific Literature
18.	ALA5107784	Binding	PROTAC activity at VHL/HDAC3 in human HCT-116 cells assessed as HDAC3 level incubated for 24 hrs by Western blot analysis relative to control	Homo sapiens	2	ALA5104222	cell-based format	Scientific Literature
19.	ALA5137975	Binding	PROTAC activity at VHL/LSD1/HDAC1/CoREST complex interaction (unknown origin) assessed as induction of HDAC1 degradation using Boc-(Ac)Lys-AMC as substrate incubated for 20 mins by fluorescence based assay	Homo sapiens	1	ALA5136981	assay format	Scientific Literature
20.	ALA5137976	Binding	PROTAC activity at VHL/HDAC1 in human HCT-116 cells assessed as induction of HDAC1 degradation at 1 uM incubated for 24 hrs by Western blot analysis relative to control	Homo sapiens	1	ALA5136981	cell-based format	Scientific Literature