# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5042901 | B | Displacement of [3H]-naloxone from mouse MOR expressed in CHO cells incubated for 1.5 hrs by competitive radioligand binding assay | Mus musculus | 5 | ALA5042476 | cell-based format | Scientific Literature | |
2. | ALA5042902 | F | Agonist activity at mouse MOR expressed in CHO cell membranes assessed as stimulation of [35S]-GTPgammaS binding measured after 1 hr by scintillation counting analysis relative to DAMGO | Mus musculus | 5 | ALA5042476 | cell membrane format | Scientific Literature | |
3. | ALA5042903 | F | Antagonist activity at mouse MOR expressed in CHO cells assessed as inhibition of DAMGO-induced calcium mobilization preincubated for 60 mins measured after 15 mins by calcium mobilization assay | Mus musculus | 5 | ALA5042476 | cell-based format | Scientific Literature | |
4. | ALA5042904 | F | Agonist activity at mouse MOR expressed in CHO cells coexpressing Gqi5 assessed as inhibition of DAMGO-induced calcium mobilization preincubated for 60 mins measured after 15 mins by calcium mobilization assay | Mus musculus | 1 | ALA5042476 | cell-based format | Scientific Literature | |
5. | ALA5042905 | B | Displacement of [125I]1-alpha from recombinant rhesus macaque CCR5 expressed in Chem-1 cells incubated for 90 mins by competitive radioligand binding assay | Macaca mulatta | 4 | ALA5042476 | cell-based format | Scientific Literature | |
6. | ALA5042907 | F | Antagonist activity at CCR5 (unknown origin) expressed in HOS cells co-expressing Gqi-5 assessed as inhibition CCL5-stimulated Ca2+ mobilization by calcium mobilization assay | Homo sapiens | 5 | ALA5042476 | cell-based format | Scientific Literature | |
7. | ALA5042908 | F | Agonist activity at CCR5 (unknown origin) expressed in HOS cells co-expressing Gqi-5 assessed as stimulation of Ca2+ mobilization by calcium mobilization assay | Homo sapiens | 2 | ALA5042476 | cell-based format | Scientific Literature | |
8. | ALA5042909 | B | Inhibition of CCR5-mediated HIV-1 Bal entry in human GHOST CCR5 cells assessed as decrease in viral reverse transcriptase activity by measuring [3H]thymidine triphosphate incorporation measured after 60 to 90 mins by scintillation counting analysis | Homo sapiens | 3 | ALA5042476 | cell-based format | Scientific Literature | |
9. | ALA5042910 | T | Cytotoxicity against human GHOST CCR5 cells assessed as reduction in cell viability measured after 6 days by XTT assay | Homo sapiens | 3 | ALA5042476 | cell-based format | Scientific Literature | |
10. | ALA5042911 | T | Therapeutic index, ratio of TC50 for human GHOST CCR5 cells to EC50 for inhibition of CCR5-mediated HIV-1 Bal entry in human GHOST CCR5 cells | 2 | ALA5042476 | cell-based format | Scientific Literature | ||
11. | ALA5042912 | B | Inhibition of MOR-mediated HIV1 BaL01 infection in GFP-tagged human OPRM1 transfected TZM-bl cells co-expressing HIV1 - LTR assessed as inhibition of viral entry by measuring LTR-driven luciferase activity pretreated for 1 hr before infection measured after 2 to 3 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature | |
12. | ALA5042913 | B | Inhibition of MOR-mediated HIV1 BaL01 infection in GFP-tagged human OPRM1 transfected TZM-bl cells co-expressing HIV1 - LTR assessed as inhibition of viral entry in presence of morphine by measuring LTR-driven luciferase activity pretreated for 1 hr before infection measured after 2 to 3 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature | |
13. | ALA5042914 | B | Inhibition of CCR5-mediated Luc-expressing HIV1 BaL Env-pseudotype virus infection in PHA-stimulated CCR5 expressing leukocytes containing human PBMC assessed as inhibition of viral entry by measuring luminescence at 100 nM pretreated for 1 hr before infection measured after 2 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature | |
14. | ALA5042915 | B | Inhibition of CCR5-mediated Luc-expressing HIV1 BaL Env-pseudotype virus infection in PHA-stimulated CCR5 expressing leukocytes containing human PBMC assessed as inhibition of viral entry in presence of 10 nM morphine by measuring luminescence at 100 nM pretreated for 1 hr before infection measured after 2 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature | |
15. | ALA5042916 | B | Inhibition of CCR5-mediated Luc-expressing HIV1 BaL Env-pseudotype virus infection in PHA-stimulated CCR5 expressing leukocytes containing human PBMC assessed as inhibition of viral entry in presence of 10 nM DMAGO by measuring luminescence at 100 nM pretreated for 1 hr before infection measured after 2 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature | |
16. | ALA5042917 | B | Inhibition of CCR5-mediated Luc-expressing HIV1 BaL Env-pseudotype virus infection in CCR5 expressing leukocytes containing human PBMC assessed as inhibition of viral entry by measuring luminescence pretreated for 1 hr before infection measured after 2 days of infection | Homo sapiens | 1 | ALA5042476 | cell-based format | Scientific Literature |