| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5029478 | B | Inhibition of truncated human N-terminal GST-fusion tagged IRAK1 (194 to 712 residues) expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay | Homo sapiens | 5 | ALA5029152 | assay format | Scientific Literature | |
| 2. | ALA5029479 | B | Inhibition of full length human N-terminal GST-fusion tagged IRAK4 expressed in baculovirus expression system using FAM-labelled peptide as substrate preincubated for 10 mins followed by substrate addition and further incubated for 1 hr in presence of ATP at Km concentration by caliper mobility shift assay | Homo sapiens | 5 | ALA5029152 | assay format | Scientific Literature | |
| 3. | ALA5029480 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at 10 uM measured after 24 hrs by Western blot analysis relative to control | Homo sapiens | 4 | ALA5029152 | cell-based format | Scientific Literature | |
| 4. | ALA5029481 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 4 | ALA5029152 | cell-based format | Scientific Literature | |
| 5. | ALA5029482 | B | Displacement of 5-FAM-labelled DEALA-HypYIPMDDDFQLRSF peptide from VHL (unknown origin) by fluorescence polarization assay | Homo sapiens | 4 | ALA5029152 | single protein format | Scientific Literature | |
| 6. | ALA5029483 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at >=1 uM measured after 2 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA5029152 | cell-based format | Scientific Literature | |
| 7. | ALA5029484 | B | Protac activity at IRAK1/VHL E3 ligase in HEK293T cells assessed as induction of IRAK1 degradation at >=1 uM measured after 8 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA5029152 | cell-based format | Scientific Literature |
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