# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5047425 | B | Inhibition of 6His tagged AURORA B (1 to 344 residue) (unknown origin) using 5FAM-GRTGRRNSI-NH2 as substrate preincubated for 30 mins followed by substrate addition and further incubated for 60 mins in presence of ATP by fluorescence polarization assay | Homo sapiens | 22 | single protein format | Scientific Literature | ||
2. | ALA5047426 | B | Inhibition of GST-fused human JAK1 (854 to 1154 residues) expressed in insect cells using ULight-JAK-1 Tyr1023 peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 20 mins in presence of ATP by FRET assay | Homo sapiens | 31 | cell-based format | Scientific Literature | ||
3. | ALA5047427 | B | Inhibition of GST-tagged recombinant human JAK2 (808 to 1132 residues) expressed in baculovirus expression system using ULight-JAK-1 Tyr1023 peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 20 mins in presence of ATP by FRET assay | Homo sapiens | 31 | assay format | Scientific Literature | ||
4. | ALA5047428 | B | Inhibition of GST-fused human JAK3 (810 to 1100 residues) expressed in insect cells using ULight-JAK-1 Tyr1023 peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 20 mins in presence of ATP by FRET assay | Homo sapiens | 31 | cell-based format | Scientific Literature | ||
5. | ALA5047429 | B | Inhibition of human TYK2 (888 to 1178 residues) expressed in insect cells using ULight-JAK-1 Tyr1023 peptide as substrate preincubated for 30 mins followed by substrate addition and further incubated for 30 mins in presence of ATP by FRET assay | Homo sapiens | 31 | cell-based format | Scientific Literature | ||
6. | ALA5047431 | B | Inhibition of human GST/6His-tagged VEGFR2 using Biotin-aminohexyl-EEEEYFELVAKKKK-NH2 as substrate incubated for 90 mins in presence of ATP by TR-FRET assay | Homo sapiens | 22 | single protein format | Scientific Literature | ||
7. | ALA5047432 | P | Solubility of the compound in stimulated lung fluid at pH 6.5 measured after 4 hrs by HPLC-UV analysis | 15 | tissue-based format | Scientific Literature | |||
8. | ALA5047433 | A | Permeability of the compound at pH 7.4 incubated for 3 hrs by HPLC based artificial membrane permeability assay | 18 | assay format | Scientific Literature | |||
9. | ALA5047434 | B | Inhibition of JAK1/JAK3 signalling in human PBMC assessed as inhibition of IL-2 stimulated IFN-gamma release incubated for 1 hr followed by IL-2 stimulation by MSD assay | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
10. | ALA5047435 | B | Inhibition of JAK1/JAK2/TYK1 in human lung fibroblast cells assessed as IL-13 induced eotaxin production incubated for 20 to 24 hrs by MSD assay | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
11. | ALA5047442 | A | Stability in human liver cytosol assessed as half life at 10 mM measured upto 60 mins in absence of AO inhibitor by LC-MS/MS analysis | Homo sapiens | 7 | subcellular format | Scientific Literature | ||
12. | ALA5047443 | A | Stability in human liver cytosol assessed as half life at 10 mM measured upto 60 mins in presence of AO inhibitor by LC-MS/MS analysis | Homo sapiens | 7 | subcellular format | Scientific Literature |