#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5031450	T	Toxicity in C57BL/6 cells assessed as effect on body weight at 10 ug measured daily for 7 days	Mus musculus	2	ALA5030531	cell-based format	Scientific Literature
2.	ALA5031451	F	Inhibition of IL-1beta release in human THP-1 derived macrophages pretreated with NLRP3 inhibitor MCC950 for 1 hr followed by compound addition and measured after 6 hrs by ELISA and immunoblotting analysis	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
3.	ALA5031452	B	Activation of NLRP3 inflammasome in human THP-1 derived macrophages assessed as increase in caspase-1 cleavage at 20 uM by immunoblotting analysis	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
4.	ALA5031453	B	Activation of NLRP3 inflammasome in human THP-1 derived macrophages assessed as increase in IL-1beta maturation and release at 20 uM measured after 6 hrs by ELISA	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
5.	ALA5031454	B	Binding affinity to NTA sensor chip immobilized recombinant human TLR4/MD2 assessed as dissociation constant by surface plasmon resonance analysis	Homo sapiens	2	ALA5030531	protein complex format	Scientific Literature
6.	ALA5031455	B	Activation of NF-kappaB in LPS-stimulated HEK-Blue hTLR4 cells assessed as increase in release of SEAP reporter protein level incubated for 18 hrs by QUANTI-blue assay	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
7.	ALA5031456	B	Activation of AP-1 in LPS-stimulated HEK-Blue hTLR4 cells assessed as increase in release of SEAP reporter protein level incubated for 18 hrs by QUANTI-blue assay	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
8.	ALA5031457	B	Antagonist activity at human TLR4 in LPS-stimulated HEK-Blue hTLR4 cells assessed as inhibition of LPS-induced SEAP production at 0.1 to 2.5 uM incubated for 18 hrs by QUANTI-blue assay	Homo sapiens	3	ALA5030531	tissue-based format	Scientific Literature
9.	ALA5031458	B	Activation of human TLR4 in IL-1beta stimulated HEK-Blue Null cells assessed as NF-kappaB activation at 0.1 to 25 uM incubated for 18 hrs by QUANTI- blue assay	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
10.	ALA5031459	B	Agonist activity human TLR2 in PAM2CSK4 stimulated HEK-Blue hTLR2 cells at 0.1 to 25 uM incubated for 18 hrs by QUANTI-blue assay	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
11.	ALA5031460	B	Activation of MyD88 dependent pathway in human THP-1 derived macrophages assessed as increase in NF-kappaB p65 phosphorylation at 10 uM measured after 1 hr by immunoblotting analysis	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
12.	ALA5031461	B	Activation of MyD88 dependent pathway in human THP-1 derived macrophages assessed as increase in MAPK p38 phosphorylation at 10 uM measured after 1 hr by immunoblotting analysis	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
13.	ALA5031462	B	Activation of MyD88 dependent pathway in human THP-1 derived macrophages assessed as increase in TNF alpha secretion at 10 to 25 uM measured after 18 hrs by ELISA	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
14.	ALA5031463	B	Activation of MyD88 dependent pathway in human THP-1 derived macrophages assessed as increase in IL-1 beta secretion at 10 to 25 uM measured after 18 hrs by ELISA	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
15.	ALA5031464	B	Activation of MyD88 dependent pathway in human THP-1 derived macrophages assessed as increase in IL-6 secretion at 10 to 25 uM measured after 18 hrs by ELISA	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
16.	ALA5031465	B	Activation of TRIF dependent pathway in human THP-1 derived macrophages assessed as increase in IRF3 phosphorylation at 10 uM measured up to 3 hrs by immunoblotting analysis	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
17.	ALA5031466	B	Activation of TRIF dependent pathway in human THP-1 derived macrophages assessed as increase in IFNbeta mRNA expression measured 2 hrs post treatment by ELISA	Homo sapiens	2	ALA5030531	tissue-based format	Scientific Literature
18.	ALA5031467	B	Activation of TRIF dependent pathway in human THP-1 derived macrophages assessed as STAT1 phosphorylation at 10 uM pretreated with IFNAR2 for 1 hr followed by compound addition and measured after 2.5 hrs by immunoblotting analysis	Homo sapiens	1	ALA5030531	tissue-based format	Scientific Literature
19.	ALA5031468	F	Immuno-adjuvant activity in C57BL/6 mouse assessed as increase in levels of anti-ova total IgG in sera at 10 ug administered subcutaneously in presence of ovalbumin and measured 21 days post immunization by ELISA	Mus musculus	2	ALA5030531	organism-based format	Scientific Literature
20.	ALA5031469	T	Toxicity in C57BL/6 cells assessed as state of alertness at 10 ug observed daily for 7 days	Mus musculus	2	ALA5030531	cell-based format	Scientific Literature