| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5054266 | B | Protac activity at VHL/BCL-XL in human MOLT-4 cells assessed as induction of BCL-XL degradation at 10 uM pretreated with VHL-032 followed by compound treatement measured after 12 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA5054267 | B | Protac activity at VHL/BCL-XL in human MOLT-4 cells assessed as induction of BCL-XL degradation at 10 uM pretreated with MG-132 followed by compound treatement measured after 12 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5054268 | B | Protac activity at VHL/BCL-XL in human MOLT-4 cells assessed as induction of BCL-XL degradation at 100 nM measured up to 72 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5054269 | U | Selectivity ratio of IC50 for human MOLT-4 cells to IC50 for platelets in human PRP | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA5054270 | B | Protac activity at VHL/BCL-XL in human platelets assessed as decrease in BCL-XL protein level at up to 1000 uM measured after 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-free format | Scientific Literature | ||
| 6. | ALA5054271 | A | Cytotoxicity against platelets in human PRP assessed as reduction in cell viability measured after 48 hrs by MTS assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 7. | ALA5054272 | B | Protac activity at VHL/BCL-XL in human RS4-11 cells assessed as decrease in BCL-XL protein level by densitometric analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 8. | ALA5054273 | B | Protac activity at VHL/BCL-XL in human MOLT-4 cells assessed as decrease in BCL-XL protein level by densitometric analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 9. | ALA5054275 | F | Cytotoxicity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by MTS assay | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
| 10. | ALA5054276 | F | Cytotoxicity against human MOLT-4 cells assessed as reduction in cell viability measured after 48 hrs by MTS assay | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
| 11. | ALA5054279 | F | Induction of apoptosis in human MOLT-4 cells assessed as decrease in cleaved caspase-3 level at up to 1000 nM measured after 16 hrs by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5054280 | F | Induction of apoptosis in human MOLT-4 cells assessed as increase in full caspase-3 level at up to 1000 nM measured after 16 hrs by immunoblotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5054281 | F | Induction of apoptosis in human MOLT-4 cells assessed as early apoptotic cells at 10 nM measured after 48 hrs by annexinV-FITC/propidium iodide staining based flow cytometry (Rvb = 2%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA5054282 | F | Induction of apoptosis in human MOLT-4 cells assessed as late apoptotic cells at 10 nM measured after 48 hrs by annexinV-FITC/propidium iodide staining based flow cytometry (Rvb = 1%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5054283 | F | Induction of apoptosis in human MOLT-4 cells assessed as early apoptotic cells at 10 nM measured after 48 hrs in presence of QVD by annexinV-FITC/propidium iodide staining based flow cytometry (Rvb = 2%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5054284 | F | Induction of apoptosis in human MOLT-4 cells assessed as late apoptotic cells at 10 nM measured after 48 hrs in presence of QVD by annexinV-FITC/propidium iodide staining based flow cytometry (Rvb = 1%) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA5054285 | B | Displacement of biotin-tagged LWAAQRYGRELRRMSDEFEGSFKGL from human BCL-XL expressed in Escherichia coli measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 18. | ALA5054286 | B | Displacement of biotin-tagged MRPEPATIAQELRRIGDEFNA from C-terminal His-tagged human BCL-2 (1` to 211 residues) expressed in Escherichia coli measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 19. | ALA5054287 | B | Binding affinity to human BCL-XL expressed in Escherichia coli/recombinant human VHL (54 to end residues) fused to human N-terminal GST-tagged elongin-C/elongin -B/N-terminal His-tagged CUL2/Rbx1 expressed in baculovirus infected Sf21 insect cells measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 20. | ALA5054288 | B | Binding affinity to C-terminal His-tagged human BCL-2 (1 to 211 residues) expressed in Escherichia coli/recombinant human VHL (54 to end residues) fused to human N-terminal GST-tagged elongin-C/elongin -B/N-terminal His-tagged CUL2/Rbx1 expressed in baculovirus infected Sf21 insect cells measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | cell-based format | Scientific Literature |
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