# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5047462 | F | Downregulation of REST protein expression in HEK293 cells at 10 uM incubated for 48 to 72 hrs by immunoblot analysis | Homo sapiens | 1 | ALA5046244 | cell-based format | Scientific Literature | |
2. | ALA5047463 | F | Downregulation of REST protein expression in HEK293 cells at 10 uM incubated for 6 hrs by immunoblot analysis relative to control | Homo sapiens | 1 | ALA5046244 | cell-based format | Scientific Literature | |
3. | ALA5047464 | F | Downregulation of REST protein expression in HEK293 cells at 10 uM incubated for 12 hrs by immunoblot analysis relative to control | Homo sapiens | 1 | ALA5046244 | cell-based format | Scientific Literature | |
4. | ALA5047465 | B | Inhibition of TCPTP (unknown origin) at 25 to 30 uM | Homo sapiens | 1 | ALA5046244 | single protein format | Scientific Literature | |
5. | ALA5047466 | B | Inhibition of wild type human SCP1 using pNPP as substrate incubated for 0.5 to 18 hrs followed by substrate addition by absorbance based analysis | Homo sapiens | 4 | ALA5046244 | single protein format | Scientific Literature | |
6. | ALA5047467 | B | Covalent inhibition of wild type human SCP1 assessed as inactivation constant using Ac-EDLphosphoSPPSPPLPK-NH2 peptide as substrate preincubated for 0.5 to 18 hrs followed by substrate addition by malachite green reagent based assay | Homo sapiens | 2 | ALA5046244 | single protein format | Scientific Literature | |
7. | ALA5047468 | B | Covalent inhibition of wild type human SCP1 assessed as inhibition constant using Ac-EDLphosphoSPPSPPLPK-NH2 peptide as substrate preincubated for 0.5 to 18 hrs followed by substrate addition by malachite green reagent based assay | Homo sapiens | 1 | ALA5046244 | single protein format | Scientific Literature | |
8. | ALA5047469 | B | Covalent inhibition of wild type human SCP1 assessed as assessed as Kinact/Ki using Ac-EDLphosphoSPPSPPLPK-NH2 peptide as substrate preincubated for 0.5 to 18 hrs followed by substrate addition by malachite green reagent based assay | Homo sapiens | 2 | ALA5046244 | single protein format | Scientific Literature | |
9. | ALA5047470 | B | Covalent inhibition of wild type human SCP1 assessed as inactivation constant using pNPP as substrate preincubated for 0.5 to 8 hrs followed by substrate addition by absorbance based analysis | Homo sapiens | 2 | ALA5046244 | single protein format | Scientific Literature | |
10. | ALA5047471 | B | Binding affinity to wild type human SCP1 assessed as covalent bond formation by measuring change in molecular mass at 500 uM incubated for overnight by MALDI-TOF analysis | Homo sapiens | 2 | ALA5046244 | single protein format | Scientific Literature | |
11. | ALA5047472 | B | Binding affinity to wild type human SCP1 assessed as protein-compound adduct formation at 500 uM incubated for overnight in presence of beta-mercaptoethanol by MALDI-TOF analysis | Homo sapiens | 2 | ALA5046244 | single protein format | Scientific Literature | |
12. | ALA5047473 | B | Covalent inhibition of human SCP1 C148A mutant using pNPP as substrate preincubated for 0.5 to 8 hrs followed by substrate addition by absorbance based analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
13. | ALA5047474 | B | Covalent inhibition of human SCP1 C92S mutant using pNPP as substrate preincubated for 0.5 to 8 hrs followed by substrate addition by absorbance based analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
14. | ALA5047475 | B | Covalent inhibition of human SCP1 C181A mutant using pNPP as substrate preincubated for 0.5 to 8 hrs followed by substrate addition by absorbance based analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
15. | ALA5047476 | B | Covalent binding affinity to human SCP1 C181A mutant assessed as covalent adduct formation incubated for overnight by MALDI-TOF analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
16. | ALA5047477 | B | Covalent binding affinity to human SCP1 C92 mutant assessed as covalent adduct formation incubated for overnight by MALDI-TOF analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
17. | ALA5047478 | B | Inhibition of human PTP1B (1 to 321 residues) expressed in Escherichia coli BL21 (DE3) using pNPP as substrate at up to 200 uM preincubated for 4 hrs followed by substrate addition by absorption based analysis | Homo sapiens | 1 | ALA5046244 | assay format | Scientific Literature | |
18. | ALA5047479 | B | Inhibition of CD45 (unknown origin) at 25 to 30 uM | Homo sapiens | 1 | ALA5046244 | single protein format | Scientific Literature | |
19. | ALA5047480 | B | Inhibition of VHR (unknown origin) at 25 to 30 uM | Homo sapiens | 1 | ALA5046244 | single protein format | Scientific Literature | |
20. | ALA5047481 | B | Inhibition of LYP (unknown origin) at 25 to 30 uM | Homo sapiens | 1 | ALA5046244 | single protein format | Scientific Literature |