# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5045984 | Binding | Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as induction of HDAC1 degradation at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 5 | ALA5042553 | cell-based format | Scientific Literature | |
2. | ALA5045985 | Binding | Protac activity at VHL/HDAC2 in human HCT-116 cells assessed as induction of HDAC2 degradation at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 6 | ALA5042553 | cell-based format | Scientific Literature | |
3. | ALA5045992 | Binding | Inhibition of HDAC2-CoREST-LSD1 (unknown origin) using Boc- (Ac)Lys-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by fluorescent assay | Homo sapiens | 5 | ALA5042553 | assay format | Scientific Literature | |
4. | ALA5045993 | Binding | Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as induction of HDAC1 degradation at 1 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 4 | ALA5042553 | cell-based format | Scientific Literature | |
5. | ALA5045994 | Binding | Protac activity at VHL/HDAC2 in human HCT-116 cells assessed as induction of HDAC2 degradation at 1 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 4 | ALA5042553 | cell-based format | Scientific Literature | |
6. | ALA5045995 | Binding | Protac activity at VHL/HDAC3 in human HCT-116 cells assessed as induction of HDAC3 degradation at 1 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 4 | ALA5042553 | cell-based format | Scientific Literature | |
7. | ALA5045996 | Binding | Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as induction of HDAC1 degradation incubated for 24 hrs by Western blot analysis | Homo sapiens | 4 | ALA5042553 | cell-based format | Scientific Literature | |
8. | ALA5045997 | Binding | Protac activity at VHL/HDAC3 in human HCT-116 cells assessed as induction of HDAC3 degradation incubated for 24 hrs by Western blot analysis | Homo sapiens | 4 | ALA5042553 | cell-based format | Scientific Literature | |
9. | ALA5045998 | Binding | Inhibition of HDAC1-CoREST-LSD1 (unknown origin) using Boc- (Ac)Lys-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by fluorescent assay | Homo sapiens | 5 | ALA5042553 | assay format | Scientific Literature | |
10. | ALA5045999 | Binding | Inhibition of HDAC3-SMRT (unknown origin) using Boc- (Ac)Lys-AMC as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by fluorescent assay | Homo sapiens | 5 | ALA5042553 | assay format | Scientific Literature | |
11. | ALA5046000 | Binding | Protac activity at VHL/HDAC1 in human HCT-116 cells assessed as increase in H3K56ac levels at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 21 | ALA5042553 | cell-based format | Scientific Literature | |
12. | ALA5046001 | Binding | Protac activity at VHL/HDAC2 in human HCT-116 cells assessed as increase in H3K56ac levels at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 20 | ALA5042553 | cell-based format | Scientific Literature | |
13. | ALA5046002 | Binding | Protac activity at VHL/HDAC3 in human HCT-116 cells assessed as increase in H3K56ac levels at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 19 | ALA5042553 | cell-based format | Scientific Literature | |
14. | ALA5046020 | Functional | Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by cell-titer glo assay | Homo sapiens | 5 | ALA5042553 | cell-based format | Scientific Literature |