# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5054769 | B | Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation at 0.001 to 10 uM measured after 6 hrs by Western blot analysis | Homo sapiens | 11 | ALA5053531 | cell-based format | Scientific Literature | |
2. | ALA5054770 | B | Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation measured after 6 hrs by Western blot analysis | Homo sapiens | 11 | ALA5053531 | cell-based format | Scientific Literature | |
3. | ALA5054771 | B | Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as maximal Brd2 degradation measured after 6 hrs by Western blot analysis relative to control | Homo sapiens | 11 | ALA5053531 | cell-based format | Scientific Literature | |
4. | ALA5054772 | B | Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation by measuring protein half life measured after 6 hrs by Western blot analysis | Homo sapiens | 3 | ALA5053531 | cell-based format | Scientific Literature | |
5. | ALA5054773 | B | Binding affinity to VHL (unknown origin) assessed as displacement of HIF-1alpha by binary binding competitive fluorescence polarization assay | Homo sapiens | 3 | ALA5053531 | single protein format | Scientific Literature | |
6. | ALA5054774 | B | Binding affinity to VHL/BRD4 BD2 domain L387A mutant (unknown origin) assessed as displacement of HIF-1alpha by ternary binding competitive fluorescence polarization assay | Homo sapiens | 3 | ALA5053531 | assay format | Scientific Literature | |
7. | ALA5054795 | B | Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation measured after 6 hrs by Western blot analysis | Homo sapiens | 11 | ALA5053531 | cell-based format | Scientific Literature | |
8. | ALA5054796 | B | Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as maximal Brd2 degradation measured after 6 hrs by Western blot analysis | Homo sapiens | 11 | ALA5053531 | cell-based format | Scientific Literature |