| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5054107 | B | Binding affinity to Beclin 1 coiled coil domain (unknown origin) by isothermal titration calorimetry assay | Homo sapiens | 18 | single protein format | Scientific Literature | ||
| 2. | ALA5054108 | B | Inhibition of FLAG-tagged Beclin 1 self-association in HEK293T cells cotransfected with GFP assessed as reduction in beclin 1- GFP pull down by FLAG-beclin 1 by coimmunoprecipitation assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5054109 | B | Induction of FLAG tagged Beclin 1- Atg14L interaction in HEK293T cells assessed as increase in endogenous beclin 1 pull down at 10 to 20 uM by coimmunoprecipitation assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5054110 | B | Induction of FLAG tagged Beclin 1- Atg14L interaction in HEK293T cells assessed as increase in endogenous beclin 1 pull down at 30 uM by coimmunoprecipitation assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5054112 | B | Induction of endolysosomal trafficking of HER2 in human SK-BR-3 cells assessed as EGF-induced degradation of HER2 at 2 uM measured after 24 hrs post-EGF stimulation by western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA5054113 | B | Induction of endolysosomal trafficking of HER2 in human SK-BR-3 cells assessed as EGF-induced degradation of HER2 at 2 uM measured after 15 hrs post-EGF stimulation by western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5054114 | B | Induction of Beclin 1- FLAG tagged UVRAG interaction in HEK293T cells assessed as endogenous beclin 1 pull down at 10 uM by coimmunoprecipitation assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA5054115 | A | Cytotoxicity against HEK293T cells assessed as reduction in cell viability at 10 to 20 uM measured after 24 hrs by trypan blue exclusion assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA5054116 | F | Induction of autophagy in HEK293T cells assessed as increase in LC3-II protein level at 10 to 20 uM incubated for 3 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5054117 | F | Induction of autophagy in human HEK293T cells assessed as increase in LC3-II protein level at 10 to 20 uM in presence of chloroquine measured after 3 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5054118 | F | Induction of autophagy in human HEK293T cells assessed as increase in p62 protein level at 10 to 20 uM measured after 3 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5054119 | F | Induction of autophagy in human HEK293T cells assessed as increase in p62 protein level at 10 to 20 uM in presence of chloroquine measured after 3 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5054120 | B | Induction of endolysosomal trafficking of EGFR in human SK-BR-3 cells assessed as EGF-induced degradation of EGFR at 2 uM measured after 15 hrs post-EGF stimulation by western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA5054123 | F | Potency index, ratio of Tat-SP4 IC50 to test compound IC50 for antiproliferative activity against human SK-BR-3 cells assessed as reduction in cell growth measured after 24 hrs by trypan blue exclusion assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 15. | ALA5054124 | F | Antiproliferative activity against human SK-BR-3 cells assessed as reduction in cell growth incubated for 5 days by trypan blue exclusion assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 16. | ALA5054126 | F | Induction of apoptosis arrest in human SK-BR-3 cells assessed as late apoptotic cells at 5 uM incubated for 24 hrs by flow cytometry with Annexin V-FITC/propidium iodide staining based flow cytometry analysis (Rvb= 2.2%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 17. | ALA5054128 | F | Induction of apoptosis in human SK-BR-3 cells assessed as increase in cell death at 10 uM in presence of Z-VAD-FMK measured after 24 hrs by trypan blue exclusion assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5054129 | F | Induction of apoptosis in human SK-BR-3 cells assessed as increase in cleaved PARP level at 10 uM incubated for 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5054131 | F | Induction of apoptosis in human SK-BR-3 cells assessed as late apoptotic cells at 10 uM incubated for 24 hrs by flow cytometry with Annexin V-FITC/propidium iodide staining based flow cytometry assay (Rvb= 2.2%) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 20. | ALA5054134 | F | Induction of apoptosis in human SK-BR-3 cells assessed as increase in cleaved caspase-3 level at 10 uM incubated for 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
The page will load shortly, Thanks for your patience!