#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5039712	B	Agonist activity at TLR2 in HEK-Blue cells incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	38	ALA5038589	cell-based format	Scientific Literature
2.	ALA5039713	B	Agonist activity at TLR2 in HEK-Blue cells assessed as fold increase in activity 0.0015 to 10 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
3.	ALA5039714	B	Agonist activity at TLR3 in HEK-Blue hTLR3 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
4.	ALA5039715	B	Agonist activity at TLR4 in HEK-Blue hTLR4 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
5.	ALA5039716	B	Agonist activity at TLR5 in HEK-Blue hTLR5 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
6.	ALA5039717	B	Agonist activity at TLR7 in HEK-Blue hTLR7 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
7.	ALA5039718	B	Agonist activity at TLR8 in HEK-Blue hTLR8 cells at 0.1 to 100 uM incubated for 24 hrs by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
8.	ALA5039719	B	Agonist activity at TLR1 in HEK-Blue hTLR2 cells at 0.2 uM incubated for 24 hrs in presence of anti-hTLR1-IgG antibodies measured by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
9.	ALA5039720	B	Agonist activity at TLR2 in HEK-Blue hTLR2 cells at 0.2 uM incubated for 24 hrs in presence of anti-hTLR2-IgA antibodies measured by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
10.	ALA5039721	B	Agonist activity at TLR6 in HEK-Blue hTLR2 cells at 0.2 uM incubated for 24 hrs in presence of anti-hTLR6-IgA antibodies measured by Quanti-Blue based SEAP assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
11.	ALA5039722	T	Cytotoxicity against HEK-Blue TLR2 cells upto 100 uM incubated for 24 hrs by CCK-8 assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
12.	ALA5039723	T	Cytotoxicity against human PBMC upto 100 uM incubated for 24 hrs by CCK-8 assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
13.	ALA5039724	T	Cytotoxicity against HEK-Blue TLR2 cells incubated for 24 hrs by CCK-8 assay	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
14.	ALA5039725	F	Selectivity index, ratio of CC50 for HEK-Blue TLR2 cells to EC50 for agonist activity at TLR2 in HEK-Blue TLR2 cells		1	ALA5038589	cell-based format	Scientific Literature
15.	ALA5039726	F	Upregulation of TLR2 expression in HEK-Blue TLR2 cells incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
16.	ALA5039727	F	Upregulation of TLR2 expression in human THP-1 cells at 0.01 to 50 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
17.	ALA5039728	F	Induction of TNFalpha secretion in TLR2 knock down human THP-1 cells at 20 to 40 uM incubated for 24 hrs by ELISA method	Homo sapiens	2	ALA5038589	cell-based format	Scientific Literature
18.	ALA5039729	B	Binding affinity to recombinant human TLR2 expressed in H5 cells by surface plasmon resonance method	Homo sapiens	2	ALA5038589	cell-based format	Scientific Literature
19.	ALA5039730	F	Upregulation of TLR1 expression in human THP-1 cells at 20 uM by immunoprecipitation method	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature
20.	ALA5039731	F	Upregulation of TLR2 expression in human THP-1 cells at 20 uM by immunoprecipitation method	Homo sapiens	1	ALA5038589	cell-based format	Scientific Literature