# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5054272 | B | Protac activity at VHL/BCL-XL in human RS4-11 cells assessed as decrease in BCL-XL protein level by densitometric analysis | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
2. | ALA5054273 | B | Protac activity at VHL/BCL-XL in human MOLT-4 cells assessed as decrease in BCL-XL protein level by densitometric analysis | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
3. | ALA5054275 | F | Cytotoxicity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by MTS assay | Homo sapiens | 13 | ALA5053511 | cell-based format | Scientific Literature | |
4. | ALA5054276 | F | Cytotoxicity against human MOLT-4 cells assessed as reduction in cell viability measured after 48 hrs by MTS assay | Homo sapiens | 13 | ALA5053511 | cell-based format | Scientific Literature | |
5. | ALA5054285 | B | Displacement of biotin-tagged LWAAQRYGRELRRMSDEFEGSFKGL from human BCL-XL expressed in Escherichia coli measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | ALA5053511 | assay format | Scientific Literature | |
6. | ALA5054286 | B | Displacement of biotin-tagged MRPEPATIAQELRRIGDEFNA from C-terminal His-tagged human BCL-2 (1` to 211 residues) expressed in Escherichia coli measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | ALA5053511 | assay format | Scientific Literature | |
7. | ALA5054287 | B | Binding affinity to human BCL-XL expressed in Escherichia coli/recombinant human VHL (54 to end residues) fused to human N-terminal GST-tagged elongin-C/elongin -B/N-terminal His-tagged CUL2/Rbx1 expressed in baculovirus infected Sf21 insect cells measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
8. | ALA5054288 | B | Binding affinity to C-terminal His-tagged human BCL-2 (1 to 211 residues) expressed in Escherichia coli/recombinant human VHL (54 to end residues) fused to human N-terminal GST-tagged elongin-C/elongin -B/N-terminal His-tagged CUL2/Rbx1 expressed in baculovirus infected Sf21 insect cells measured after 2 hrs by AlphaScreen assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
9. | ALA5054292 | B | Binding affinity to Halo-tagged VHL/HiBiT-tagged BCL-XL (unknown origin) expressed in HEK293T cells assessed as ternary complex formation at up to 100000 nM measured after 6 hrs by NanoBRET assay | Homo sapiens | 5 | ALA5053511 | cell-based format | Scientific Literature | |
10. | ALA5054293 | B | Binding affinity to Halo-tagged VHL/HiBiT-tagged BCL-2 (unknown origin) expressed in HEK293T cells assessed as ternary complex formation at up to 100000 nM measured after 6 hrs by NanoBRET assay | Homo sapiens | 5 | ALA5053511 | cell-based format | Scientific Literature | |
11. | ALA5054294 | F | Cytotoxicity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs in presence of VHL-032 by MTS assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
12. | ALA5054295 | B | Binding affinity to VHL/ BCL-XL in human RS4-11 cells assessed as ternary complex formation at up to 300 nM measured after 12 hrs by immunoprecipitation assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
13. | ALA5054296 | B | Binding affinity to VHL/BCL-2 in human RS4-11 cells assessed as ternary complex formation at up to 300 nM measured after 12 hrs by immunoprecipitation assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
14. | ALA5054297 | B | Binding affinity to VHL/ BCL-XL in human RS4-11 cells assessed as ternary complex formation at up to 300 nM measured after 12 hrs in presence of VHL-032 by immunoprecipitation assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature | |
15. | ALA5054298 | B | Binding affinity to VHL/BCL-2 in human RS4-11 cells assessed as ternary complex formation at up to 300 nM measured after 12 hrs in presence of VHL-032 by immunoprecipitation assay | Homo sapiens | 4 | ALA5053511 | cell-based format | Scientific Literature |