#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5054769	B	Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation at 0.001 to 10 uM measured after 6 hrs by Western blot analysis	Homo sapiens	11		cell-based format	Scientific Literature
2.	ALA5054770	B	Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation measured after 6 hrs by Western blot analysis	Homo sapiens	11		cell-based format	Scientific Literature
3.	ALA5054771	B	Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as maximal Brd2 degradation measured after 6 hrs by Western blot analysis relative to control	Homo sapiens	11		cell-based format	Scientific Literature
4.	ALA5054772	B	Bump and hole PROTAC activity at BromoTag-Brd2/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation by measuring protein half life measured after 6 hrs by Western blot analysis	Homo sapiens	3		cell-based format	Scientific Literature
5.	ALA5054773	B	Binding affinity to VHL (unknown origin) assessed as displacement of HIF-1alpha by binary binding competitive fluorescence polarization assay	Homo sapiens	3		single protein format	Scientific Literature
6.	ALA5054774	B	Binding affinity to VHL/BRD4 BD2 domain L387A mutant (unknown origin) assessed as displacement of HIF-1alpha by ternary binding competitive fluorescence polarization assay	Homo sapiens	3		assay format	Scientific Literature
7.	ALA5054775	F	Antiproliferative activity against human BET-sensitive MV4-11 cells assessed as reduction in cell viability measured after 24 hrs by Celltiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA5054778	B	Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation measured in presence of MLN4924 after 6 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
9.	ALA5054779	B	Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation measured in presence of VH298 after 6 hrs by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
10.	ALA5054780	B	Reversibility of Bump and hole PROTAC activity at BromoTag-Brd2 BD2 L387A mutant/VHL-bound E3 ligase in HEK293 cells assessed as Brd2 degradation at 200 nM treated for 3 hrs measured after 24 hrs of compound washout by Western blot analysis	Homo sapiens	1		cell-based format	Scientific Literature
11.	ALA5054781	A	Stability in mouse plasma measured up to 60 mins	Mus musculus	1		cell-free format	Scientific Literature
12.	ALA5054782	A	Clearance in C57BL/6 mouse at 5 mg/kg, iv measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
13.	ALA5054783	A	Volume of distribution at steady state in C57BL/6 mouse at 5 mg/kg, iv measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
14.	ALA5054784	A	Half life in C57BL/6 mouse at 5 mg/kg, iv measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
15.	ALA5054785	A	Half life in C57BL/6 mouse at 5 mg/kg, sc measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
16.	ALA5054786	A	AUC (0 to last) in C57BL/6 mouse at 5 mg/kg, iv measured up to 8 hrs by LC-MS analysis	Mus musculus	1		organism-based format	Scientific Literature
17.	ALA5054787	A	AUC (0 to last) in C57BL/6 mouse at 5 mg/kg, sc measured up to 8 hrs by LC-MS analysis	Mus musculus	1		organism-based format	Scientific Literature
18.	ALA5054788	A	AUC (0 to infinity) in C57BL/6 mouse at 5 mg/kg, iv measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
19.	ALA5054789	A	AUC (0 to infinity) in C57BL/6 mouse at 5 mg/kg, sc measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature
20.	ALA5054790	A	MRT (0 to infinity) in C57BL/6 mouse at 5 mg/kg, sc measured up to 8 hrs by LC-MS analysis	Mus musculus	2		organism-based format	Scientific Literature