| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5038998 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of RbAp48 degradation at 1 uM incubated for 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA5038999 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of EED degradation at 1 uM incubated for 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5039000 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of SUZ12 degradation at 1 uM incubated for 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5039001 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of EZH2 degradation at 1 uM incubated for 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5039003 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of RbAp48 degradation at 1 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
| 6. | ALA5039004 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of SUZ12 degradation at 1 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
| 7. | ALA5039005 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of EED degradation at 1 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
| 8. | ALA5039006 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of EZH2 degradation at 1 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
| 9. | ALA5039007 | B | Binding affinity to RbAp48 in human WSUDLCL2 cells assessed as thermal stability by measuring shift in temperature at 60 uM pretreated with proteasome inhibitor MG132 for 1 hr followed by compound addition incubated for 2 hrs by cellular thermal shift based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5039008 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as reduction in H3K27me3 level incubated for 48 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5039009 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as downregulation of RbAp48 expression at 1 uM pretreated with EPZ6438 for 4 hrs followed by compound addition and measured after 48 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5039016 | B | Inhibition of N-terminal His-tagged EZH2 in human PRC2 complex (2 to end residues) expressed in Sf9 cells using [3H]-SAM as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by AlphaLISA immunodetection assay | Homo sapiens | 22 | cell-based format | Scientific Literature | ||
| 13. | ALA5039017 | F | Downregulation of CENPK mRNA expression in human NCI-H1299 cells at 10 uM incubated for 48 hrs by qRT-PCR analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 14. | ALA5039018 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as reduction in H3K27me3 level at 1 uM incubated for 48 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5039019 | B | Binding affinity to EZH2 in human WSUDLCL2 cells assessed as thermal stability by measuring shift in temperature at 60 uM pretreated with proteasome inhibitor MG132 for 1 hr followed by compound addition incubated for 2 hrs by cellular thermal shift based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5039020 | F | Antiproliferative activity against human WSUDLCL2 cells harboring EZH2-Y641F mutant assessed as inhibition of cell growth at 10 uM incubated for 9 days by optical microscopic analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 17. | ALA5039021 | B | Binding affinity to EED in human WSUDLCL2 cells assessed as thermal stability by measuring shift in temperature at 60 uM pretreated with proteasome inhibitor MG132 for 1 hr followed by compound addition incubated for 2 hrs by cellular thermal shift based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5039022 | B | Binding affinity to SUZ12 in human WSUDLCL2 cells assessed as thermal stability by measuring shift in temperature at 60 uM pretreated with proteasome inhibitor MG132 for 1 hr followed by compound addition incubated for 2 hrs by cellular thermal shift based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5039023 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of EZH2 degradation incubated for 48 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA5039024 | B | PROTAC activity at CRBN E3 ligase/EZH2 in PRC2 complex in human WSUDLCL2 cells assessed as induction of SUZ12 degradation incubated for 48 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
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