# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5031410 | Binding | Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells using tetrahydrofolate as substrate preincubated for 10 min in presence of NAD+ followed by addition of substrate and further incubated for 10 mins | Homo sapiens | 11 | ALA5030528 | cell-based format | Scientific Literature | |
2. | ALA5031411 | Binding | Inhibition of C-terminal His-tagged human MTHFD1 (1 to 306 residues) expressed in insect cells using tetrahydrofolate as substrate preincubated for 10 min in presence of NAD+ followed by addition of substrate and further incubated for 10 mins | Homo sapiens | 11 | ALA5030528 | cell-based format | Scientific Literature | |
3. | ALA5031412 | Binding | Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells catalytic activity using varying levels of tetrahydrofolate as substrate | Homo sapiens | 3 | ALA5030528 | cell-based format | Scientific Literature | |
4. | ALA5031415 | Binding | Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Km by using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis | Homo sapiens | 2 | ALA5030528 | cell-based format | Scientific Literature | |
5. | ALA5031416 | Binding | Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Vmax using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis | Homo sapiens | 2 | ALA5030528 | cell-based format | Scientific Literature |