# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5043414 | Binding | Binding affinity to recombinant N-terminal His6-tagged human STK3 (18 to 311 residues) expressed in Escherichia coli assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 2 | ALA5042489 | assay format | Scientific Literature | |
2. | ALA5043415 | Binding | Binding affinity to recombinant N-terminal His6-tagged human STK4 (43 to 431 residues) expressed in Escherichia coli assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 2 | ALA5042489 | assay format | Scientific Literature | |
3. | ALA5043416 | Binding | Inhibition of recombinant N-terminal His6-tagged human STK4 (43 to 431 residues) expressed in Escherichia coli assessed as melting temperature at 20 uM by thermal shift assay (Rvb = 55 degreeC) | Homo sapiens | 3 | ALA5042489 | assay format | Scientific Literature | |
4. | ALA5043419 | Binding | Inhibition of recombinant N-terminal His6-tagged human STK4 (43 to 431 residues) expressed in Escherichia coli using myelin basic protein as substrate in presence of ATP by ADP-Glo luminescence kinase assay | Homo sapiens | 32 | ALA5042489 | assay format | Scientific Literature | |
5. | ALA5043420 | Binding | Inhibition of recombinant N-terminal His6-tagged human STK3 (18 to 311 residues) expressed in Escherichia coli using myelin basic protein as substrate in presence of ATP by ADP-Glo luminescence kinase assay | Homo sapiens | 32 | ALA5042489 | assay format | Scientific Literature | |
6. | ALA5043423 | Binding | Binding affinity to recombinant N-terminal His6-tagged human STK3 (18 to 311 residues) expressed in Escherichia coli assessed as inhibition constant | Homo sapiens | 3 | ALA5042489 | assay format | Scientific Literature | |
7. | ALA5043424 | Binding | Binding affinity to recombinant N-terminal His6-tagged human STK4 (43 to 431 residues) expressed in Escherichia coli assessed as inhibition constant | Homo sapiens | 3 | ALA5042489 | assay format | Scientific Literature | |
8. | ALA5043425 | Binding | Displacement of K5 tracer from full length C-terminal NanoLuc fused human STK3 expressed in HEK293T cells incubated for 2 hrs by NanoBRET assay | Homo sapiens | 14 | ALA5042489 | cell-based format | Scientific Literature | |
9. | ALA5043426 | Binding | Inhibition of recombinant N-terminal His6-tagged human STK3 (18 to 311 residues) expressed in Escherichia coli assessed as melting temperature at 20 uM by thermal shift assay (Rvb = 56.8 degreeC) | Homo sapiens | 6 | ALA5042489 | assay format | Scientific Literature | |
10. | ALA5043427 | Binding | Displacement of K5 tracer from full length C-terminal NanoLuc fused human STK4 expressed in HEK293T cells incubated for 2 hrs by NanoBRET assay | Homo sapiens | 14 | ALA5042489 | cell-based format | Scientific Literature | |
11. | ALA5043428 | Binding | Displacement of K5 tracer from full length C-terminal NanoLuc fused human LRRK2 expressed in HEK293T cells incubated for 2 hrs by NanoBRET assay | Homo sapiens | 13 | ALA5042489 | cell-based format | Scientific Literature | |
12. | ALA5043429 | Binding | Inhibition of STK3 in HEK293 cells assessed as reduction in phosphorylated-MOB1 protein level at 10 uM preincubated for 4 hrs followed by H2O2 addition and measured after 2 hrs by Western blotting analysis | Homo sapiens | 14 | ALA5042489 | cell-based format | Scientific Literature | |
13. | ALA5043430 | Binding | Inhibition of STK3 in HEK293 cells assessed as increase in YAP/TAZ-TEAD activity at 10 uM incubated for 48 hrs by Dual-Glo luciferase assay | Homo sapiens | 7 | ALA5042489 | cell-based format | Scientific Literature | |
14. | ALA5043431 | Binding | Inhibition of STK4 in HEK293 cells assessed as increase in YAP/TAZ-TEAD activity at 10 uM incubated for 48 hrs by Dual-Glo luciferase assay | Homo sapiens | 7 | ALA5042489 | cell-based format | Scientific Literature | |
15. | ALA5043432 | ADME | Drug concentration in C57BL/6 mouse plasma at 20 mg/kg, ip measured after 30 mins by LC-MS/MS analysis | Mus musculus | 14 | ALA5042489 | organism-based format | Scientific Literature | |
16. | ALA5043455 | Binding | Inhibition of STK4 in HEK293 cells assessed as reduction in phosphorylated-MOB1 protein level at 10 uM preincubated for 4 hrs followed by H2O2 addition and measured after 2 hrs by Western blotting analysis | Homo sapiens | 14 | ALA5042489 | cell-based format | Scientific Literature |