#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5031410	Binding	Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells using tetrahydrofolate as substrate preincubated for 10 min in presence of NAD+ followed by addition of substrate and further incubated for 10 mins	Homo sapiens	11	ALA5030528	cell-based format	Scientific Literature
2.	ALA5031411	Binding	Inhibition of C-terminal His-tagged human MTHFD1 (1 to 306 residues) expressed in insect cells using tetrahydrofolate as substrate preincubated for 10 min in presence of NAD+ followed by addition of substrate and further incubated for 10 mins	Homo sapiens	11	ALA5030528	cell-based format	Scientific Literature
3.	ALA5031412	Binding	Inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells catalytic activity using varying levels of tetrahydrofolate as substrate	Homo sapiens	3	ALA5030528	cell-based format	Scientific Literature
4.	ALA5031415	Binding	Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Km by using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	2	ALA5030528	cell-based format	Scientific Literature
5.	ALA5031416	Binding	Uncompetitive inhibition of C-terminal His-tagged human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as decrease in Vmax using varying levels of tetrahydrofolate as substrate by double-reciprocal plot analysis	Homo sapiens	2	ALA5030528	cell-based format	Scientific Literature
6.	ALA5031417	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as induction of conformational changes	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
7.	ALA5031418	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as induction of betae-alphaE loop shift in monomer A	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
8.	ALA5031419	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as destabilization of betae-alphaE loop shift in monomer A	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature
9.	ALA5031420	Binding	Binding affinity to human MTHFD2 (36 to 350 residues) expressed in insect cells assessed as movement of alphaD2-alphaD3 loop in monomer B	Homo sapiens	1	ALA5030528	cell-based format	Scientific Literature