# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5031971 | A | Cytotoxicity against African green monkey COS-1 cell assessed as reduction in cell viability incubated for 24 hrs by by CellTiter 96 non-radioactive cell proliferation assay | Chlorocebus aethiops | 6 | cell-based format | Scientific Literature | ||
2. | ALA5031972 | A | Drug uptake in cytoplasm of African green monkey COS-1 cells assessed as compound accumulation in cytoplasm at 20 uM incubated for 4 hrs in presence of FITC-labelled peptide by DAPI staining based inverted phase contrast fluorescence microscope analysis | Chlorocebus aethiops | 5 | subcellular format | Scientific Literature | ||
3. | ALA5031973 | A | Drug uptake in nucleus of African green monkey COS-1 cells assessed as compound accumulation in nucleus at 20 uM incubated for 4 hrs in presence of FITC-labelled peptide by DAPI staining based inverted phase contrast fluorescence microscope analysis | Chlorocebus aethiops | 5 | subcellular format | Scientific Literature | ||
4. | ALA5031978 | B | Inhibition of GRTH phosphorylation in mouse seminiferous tubule of testis assessed as reduction in pGRTH protein level at 100 uM measured after 16 hrs by western blot analysis | Mus musculus | 3 | tissue-based format | Scientific Literature | ||
5. | ALA5031979 | B | Binding affinity to GRTH (unknown origin) expressed in COS-1 cell lysate assessed as thermostabilization of protein at 40 uM incubated for 16 hrs followed by heating at 40 to 70 degree C for 3 mins by CETSA based western blot analysis | Homo sapiens | 3 | subcellular format | Scientific Literature | ||
6. | ALA5031980 | B | Binding affinity to mCherry labelled GRTH (unknown origin) expressed in COS-1 cells assessed as increase in fluorescence intensity of donor peptide FITC in bleached region at 15 uM followed by acceptor photobleaching measured after 16 hrs by inverted microscope based live cell imaging analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
7. | ALA5031981 | B | Inhibition of PKA-induced GRTH phosphorylation (unknown origin) expressed in COS-1 cells at 100 uM stimulated with PKA for 24 hrs followed by peptide addition and incubated overnight by Western blot analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
8. | ALA5031982 | B | Inhibition of GRTH phosphorylation (unknown origin) expressed in COS-1 cells assessed as reduction in pGRTH protein level at 5 to 100 uM measured after 16 hrs by western blot analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
9. | ALA5031983 | A | Drug uptake in mouse seminiferous tubule of testis at 100 uM incubated for 16 hrs by immunofluorescence microscopic analysis | Mus musculus | 5 | tissue-based format | Scientific Literature | ||
10. | ALA5031985 | B | Binding affinity to mCherry labelled GRTH (unknown origin) expressed in COS-1 cells assessed as FRET efficiency at 15 uM followed by acceptor photobleaching measured after 16 hrs by inverted microscope based live cell imaging analysis relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature |