# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5042938 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as reduction in EZH2 level at 2 uM preincubated with VH032 for 2 hrs followed by compound addition and measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
2. | ALA5042943 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as reduction in H3K27me3 level at 1 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 5 | ALA5042478 | cell-based format | Scientific Literature | |
3. | ALA5042950 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as EZH2 protein abundance after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
4. | ALA5042951 | B | Protac activity at VHL/EZH1 in human 22Rv1 cells assessed as EZH1 protein abundance at 1 to 6 uM after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
5. | ALA5042952 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as maximum degradation efficacy at 2 uM by Western blot analysis relative to control | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
6. | ALA5042953 | B | Protac activity at VHL/EED in human 22Rv1 cells assessed as reduction in EED level at 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
7. | ALA5042954 | B | Protac activity at VHL/SUZ12 in human 22Rv1 cells assessed as reduction in SUZ12 level at 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
8. | ALA5042955 | B | Protac activity at VHL/EZH2 in VHL knockdown human 22Rv1 cells assessed as reduction in EZH2 level at 2 uM for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
9. | ALA5042956 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as increase in EZH2 ubiquitination at 2 uM for 12 hrs by immunoprecipitation based Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
10. | ALA5042957 | F | Antiproliferative activity against human SUDHL2 cells assessed as reduction in cell viability at 0.01 to 10 uM measured after 5 days by MTS assay | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
11. | ALA5042958 | F | Antiproliferative activity against human SU-DHL-4 cells assessed as reduction in cell viability at 0.01 to 10 uM measured after 5 days by MTS assay | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
12. | ALA5042959 | F | Antiproliferative activity against human SU-DHL-6 cells assessed as reduction in cell viability at 0.01 to 10 uM measured after 5 days by MTS assay | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
13. | ALA5042960 | B | Induction of EZH2 degradation in human SUDHL2 cells at 0.1 to 5 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
14. | ALA5042962 | F | Induction of cell cycle arrest in human SU-DHL-6 cells assessed as accumulation of cells at sub G1 phase after 24 hrs by flow cytometry | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
15. | ALA5042963 | F | Induction of apoptosis in human SU-DHL-6 cells assessed as increase in cleaved PARP level at 1 to 5 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
16. | ALA5042964 | F | Induction of apoptosis in human SU-DHL-6 cells assessed as increase in cleaved capase-3 level at 1 to 5 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
17. | ALA5042965 | F | Induction of apoptosis in human SU-DHL-6 cells at 1 to 5 uM incubated for 48 hrs by Annexin V/propidium iodide staining based flow cytometry | Homo sapiens | 3 | ALA5042478 | cell-based format | Scientific Literature | |
18. | ALA5042966 | B | Protac activity at VHL/EZH2 in human 22Rv1 cells assessed as reduction in H3K27me3 level after 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5042478 | cell-based format | Scientific Literature | |
19. | ALA5042975 | B | Protac activity at VHL/SUZ12 in human SUDHL2 cells assessed as reduction in SUZ12 level at 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature | |
20. | ALA5042976 | B | Protac activity at VHL/EED in human SUDHL2 cells assessed as reduction in EED level at 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5042478 | cell-based format | Scientific Literature |