Antitubercular activity against Mycobacterium smegmatis mc2155 ATCC 700084 cultured in GAST-Fe media incubated for 24 hrs by resazurin microtiter assay
Selectivity index , ratio of MIC90 in Mycobacterium smegmatis mc2155 ATCC 700084 cultured in GAST-Fe media to Mycobacterium smegmatis mc2155 cultured in GAST media
Antitubercular activity against Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in GAST-Fe media incubated for 8 days by resazurin microtiter assay
Selectivity index , ratio of MIC90 in Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in GAST-Fe media to Mycobacterium tuberculosis H37Rv ATCC 27294 cultured in GAST media
Selectivity index , ratio of MIC90 in Mycobacterium aurum ATCC 10437 cultured in GAST-Fe media to Mycobacterium aurum ATCC 10437 cultured in GAST media
Selectivity index , ratio of MIC90 in Mycobacterium bovis BCG ATCC 35734 cultured in GAST-Fe media to Mycobacterium bovis BCG ATCC 35734 cultured in GAST media
Bacteriostatic activity against Mycobacterium smegmatis mc2155 ATCC 700084 assessed as inhibition of bacterial growth preincubated for 48 hrs followed by agar surface scraped and transfer to GAST medium and measured after 5 days by HT-SPOTi assay
Antibacterial activity against Mycobacterium smegmatis mc2155 ATCC 700084 cultured in iron supplemented medium assessed as inhibition of siderophore production at 4 ug/ml measured after 3 hrs by CAS assay relative to control
Binding affinity to Mycobacterium tuberculosis MbtA assessed as change in thermal stability at 100 uM incubated for 1 hr in presence of ATP at pH 7.5 by thermo fluorometer analysis
Binding affinity to Mycobacterium tuberculosis MbtA assessed as change in thermal stability at 100 uM incubated for 1 hr in presence of ATP at pH 8.5 by thermo fluorometer analysis
Antibacterial activity against Mycobacterium smegmatic mc2155 ATCC 700084 assessed as inhibition of efflux pump using ethidium bromide as substrate measured every min for 45 mins
Selectivity index, ratio of GIC90 for cytotoxicity against mouse RAW264.7 cells to MIC90 for Antitubercular activity against Mycobacterium smegmatis mc2155 ATCC 700084