# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5043741 | Binding | Inhibition of RSK1 kinase (unknown origin) at 0.1 uM | Homo sapiens | 2 | ALA5042500 | single protein format | Scientific Literature | |
2. | ALA5043742 | Physicochemical | Solubility of the compound in FeSSIF at pH 5.0 | 3 | ALA5042500 | small-molecule physicochemical format | Scientific Literature | ||
3. | ALA5043743 | ADME | Inhibition of CYP3A4 in human liver microsomes using testosterone as substrate incubated for 30 mins by LC-MS/MS analysis | Homo sapiens | 4 | ALA5042500 | microsome format | Scientific Literature | |
4. | ALA5043744 | ADME | Metabolic stability in CD1 mouse liver microsomes assessed as compound degradation in presence of NADPH measured after 20 mins | Mus musculus | 4 | ALA5042500 | microsome format | Scientific Literature | |
5. | ALA5043745 | Binding | Inhibition of recombinant human SGK1 expressed in baculovirus expression system using 5-carboxyfluorescein -RPRAATF-NH2 as substrate preincubated for 30 mins followed by substrate addition in presence of 10 uM ATP and measured after 60 mins by microfluidics assay | Homo sapiens | 54 | ALA5042500 | assay format | Scientific Literature | |
6. | ALA5043746 | Binding | Inhibition of recombinant human SGK1 expressed in baculovirus expression system using 5-carboxyfluorescein -RPRAATF-NH2 as substrate preincubated for 30 mins followed by substrate addition in presence of 500 uM ATP and measured after 60 mins by microfluidics assay | Homo sapiens | 56 | ALA5042500 | assay format | Scientific Literature | |
7. | ALA5043750 | Binding | Inhibition of mouse SGK1 in presence 500 uM presence of ATP | Mus musculus | 4 | ALA5042500 | single protein format | Scientific Literature | |
8. | ALA5043751 | Binding | Inhibition of rat SGK1 in presence 500 uM presence of ATP | Rattus norvegicus | 4 | ALA5042500 | assay format | Scientific Literature | |
9. | ALA5043752 | Physicochemical | Distribution coefficient, logD of compound at pH 7.4 by chromatographic analysis | 4 | ALA5042500 | small-molecule physicochemical format | Scientific Literature | ||
10. | ALA5043753 | ADME | Permeability in human Caco2 cells at 20 uM incubated for 120 mins in presence of cyclosporin by ESI-MS/MS analysis | Homo sapiens | 4 | ALA5042500 | cell-based format | Scientific Literature | |
11. | ALA5043754 | Physicochemical | Aqueous solubility of the compound at pH 7.4 | 4 | ALA5042500 | small-molecule physicochemical format | Scientific Literature | ||
12. | ALA5043755 | Functional | Effect on chondrocyte hypertrophic differentiation mouse ATDC-5 cells assessed as inhibition of Col2a1 gene expression incubated for 2 weeks followed by compound added and every 3 days medium is changed measured after 2 weeks by RT-qPCR analysis | Mus musculus | 4 | ALA5042500 | cell-based format | Scientific Literature | |
13. | ALA5043756 | ADME | Permeability in human Caco2 cells at 20 uM incubated for 120 mins by ESI-MS/MS analysis | Homo sapiens | 4 | ALA5042500 | cell-based format | Scientific Literature | |
14. | ALA5043757 | Physicochemical | Chemical stability of the compound at pH 7.4 measured after 24 hrs | 4 | ALA5042500 | small-molecule physicochemical format | Scientific Literature | ||
15. | ALA5043758 | ADME | Metabolic stability in human liver microsomes assessed as compound degradation in presence of NADPH measured after 20 mins | Homo sapiens | 4 | ALA5042500 | microsome format | Scientific Literature | |
16. | ALA5043759 | ADME | Metabolic stability in Sprague-Dawley rat liver microsomes assessed as compound degradation in presence of NADPH measured after 20 mins | Rattus norvegicus | 4 | ALA5042500 | microsome format | Scientific Literature | |
17. | ALA5043760 | ADME | Intrinsic clearance in human hepatocytes assessed per 10^-6 cells | Homo sapiens | 4 | ALA5042500 | cell-based format | Scientific Literature | |
18. | ALA5043761 | ADME | Inhibition of CYP3A4 in human liver microsomes using midazolam as substrate incubated for 10 mins by LC-MS/MS analysis | Homo sapiens | 4 | ALA5042500 | microsome format | Scientific Literature | |
19. | ALA5043762 | ADME | Inhibition of CYP3A4 in human liver microsomes by measuring maximum efficacy | Homo sapiens | 4 | ALA5042500 | microsome format | Scientific Literature | |
20. | ALA5043763 | Toxicity | Inhibition of human ERG at 1 uM relative to control | Homo sapiens | 4 | ALA5042500 | assay format | Scientific Literature |