# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5123586 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
2. | ALA5123587 | B | Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay | Mus musculus | 8 | cell-based format | Scientific Literature | ||
3. | ALA5123590 | B | Inhibition of human recombinant LDHA using pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 10 | single protein format | Scientific Literature | ||
4. | ALA5123591 | B | Inhibition of human recombinant LDHB using pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 9 | single protein format | Scientific Literature | ||
5. | ALA5123608 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Agxt1 knockdown PH1 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of glycolate relative to control | Mus musculus | 9 | cell-based format | Scientific Literature | ||
6. | ALA5123610 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Grhpr-knockdown PH2 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of glycolate relative to control | Mus musculus | 9 | cell-based format | Scientific Literature | ||
7. | ALA5123611 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Grhpr-knockdown PH2 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 48 hrs in the presence of glycolate relative to control | Mus musculus | 9 | cell-based format | Scientific Literature | ||
8. | ALA5123612 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Hoga1-knockdown PH3 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of hydroxyproline relative to control | Mus musculus | 9 | cell-based format | Scientific Literature | ||
9. | ALA5123613 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Hoga1-knockdown PH3 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 48 hrs in the presence of hydroxyproline relative to control | Mus musculus | 9 | cell-based format | Scientific Literature | ||
10. | ALA5123614 | T | Cytotoxicity against hyperoxaluric-primary hepatocyte isolated from Hoga1-knockdown PH3 C57BL/6 mouse assessed as cell death at 10 uM by MTS assay | Mus musculus | 10 | cell-based format | Scientific Literature | ||
11. | ALA5123615 | T | Cytotoxicity against hyperoxaluric-primary hepatocyte isolated from Grhpr-knockdown PH2 C57BL/6 mouse assessed as cell death at 10 uM by MTS assay | Mus musculus | 10 | cell-based format | Scientific Literature | ||
12. | ALA5123616 | T | Cytotoxicity against hyperoxaluric-primary hepatocyte isolated from Agxt1-knockdown PH1 C57BL/6 mouse assessed as cell death at 10 uM by MTS assay | Mus musculus | 10 | cell-based format | Scientific Literature | ||
13. | ALA5123631 | F | Antiinflammatory activity against LPS-stimulated human THP-1 cells assessed as reduction in TNFalpha level preincubated with compound for 24 hrs followed by LPS stimulation for 24 hrs by ELISA analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
14. | ALA5123632 | F | Antiinflammatory activity against LPS-stimulated human THP-1 cells assessed as reduction in IL6 level preincubated with compound for 24 hrs followed by LPS stimulation for 24 hrs by ELISA analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
15. | ALA5123633 | F | Antiinflammatory activity against LPS-stimulated human THP-1 cells assessed as reduction in IL-1beta level preincubated with compound for 24 hrs followed by LPS stimulation for 24 hrs by ELISA analysis | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
16. | ALA5123634 | T | Cytotoxicity against HEK293 cells assessed as reduction in cell viability upto 50 uM measured after 48 hrs by MTT assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
17. | ALA5123635 | F | Cytotoxicity against human 786-0 cells assessed as reduction in cell viability upto 100 uM measured after 48 hrs by MTT assay | Homo sapiens | 8 | cell-based format | Scientific Literature |