| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5100168 | B | Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 2. | ALA5100169 | B | Inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 3. | ALA5100170 | B | Selectivity index, ratio of IC50 for inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 4. | ALA5100171 | B | Inhibition of recombinant human C-terminal FLAG-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA5100172 | B | Inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in Sf9 cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 6. | ALA5100173 | B | Inhibition of recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus infected Sf9 insect cells using Boc-Lys (trifluoroacetyl)-AMC as substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 7. | ALA5100174 | B | Selectivity index, ratio of IC50 for inhibition of recombinant human C-terminal FLAG-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 8. | ALA5100175 | B | Selectivity index, ratio of IC50 for inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in Sf9 cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 9. | ALA5100176 | B | Selectivity index, ratio of IC50 for inhibition fo recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus infected Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA5100177 | F | Cytotoxicity against human HAL-01 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 11. | ALA5100178 | F | Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 12. | ALA5100179 | F | Cytotoxicity against human Jurkat cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 13. | ALA5100180 | F | Induction of apoptosis in human HL-60 cells assessed as increase in total apoptotic cells at 1 to 5 uM incubated for 48 hrs by annexin V and propidium iodide staining based flow cytometry analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 14. | ALA5100189 | B | Inhibition of HDAC in human HL-60 cells assessed as increase in alpha tubulin acetylation at 6 to 36 uM incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5100190 | B | Inhibition of HDAC in human HL-60 cells assessed as increase in Ac-H3 levels at <=36 uM incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
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