# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5167459 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT at 3 uM incubated for 48 hrs | Homo sapiens | 7 | ALA5154920 | cell-based format | Scientific Literature | |
2. | ALA5167460 | F | Antiproliferative activity against human A2780 cells overexpressing NAMPT assessed as cell growth inhibition incubated for 72 hrs | Homo sapiens | 2 | ALA5154920 | cell-based format | Scientific Literature | |
3. | ALA5167461 | F | Antiproliferative activity against human HCT-116 cells expressing NAMPT assessed as cell growth inhibition incubated for 72 hrs | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
4. | ALA5167463 | B | Binding affinity to NAMPT in human A2780 cell lysate assessed as thermal stability incubated at 51 to 63 degree celcius for 4 hrs by Western blotting based CETSA method | Homo sapiens | 1 | ALA5154920 | subcellular format | Scientific Literature | |
5. | ALA5167467 | B | Inhibition of NAMPT (unknown origin) measured after 90 mins by measuring fluorescence intensity | Homo sapiens | 8 | ALA5154920 | single protein format | Scientific Literature | |
6. | ALA5167468 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT at 0.1 uM incubated for 48 hrs | Homo sapiens | 7 | ALA5154920 | cell-based format | Scientific Literature | |
7. | ALA5167469 | B | Binding affinity to LC3B (unknown origin) assessed as dissociation constant by surface plasmon resonance analysis | Homo sapiens | 1 | ALA5154920 | single protein format | Scientific Literature | |
8. | ALA5167470 | B | Autophagosome tethering activity at KSP overexpressing human A2780 cells assessed as lysosome-mediated degradation of KSP at 0.03 to 10 uM | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
9. | ALA5167471 | F | Antiproliferative activity against human MDA-MB-231 cells overexpressing NAMPT assessed as cell growth inhibition incubated for 72 hrs | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
10. | ALA5167472 | P | Stability of compound in 1640 culture medium measured up to 72 hrs by HPLC analysis | 1 | ALA5154920 | small-molecule physicochemical format | Scientific Literature | ||
11. | ALA5167473 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT by measuring protein level at 500 nM after 48 hrs in presence of MS2 by western blot analysis | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
12. | ALA5167474 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT by measuring protein level at 500 nM measured after 48 hrs by western blot analysis | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
13. | ALA5167476 | B | Binding affinity to KSP in human A2780 cell lysate incubated at 51 to 63 degree celcius for 4 hrs by Western blotting based CETSA method | Homo sapiens | 1 | ALA5154920 | subcellular format | Scientific Literature | |
14. | ALA5167477 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT by measuring protein level at 500 nM after 48 hrs in presence of FK866 by western blot analysis | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature | |
15. | ALA5167479 | B | Autophagosome tethering activity at NMAPT overexpressing human A2780 cells assessed as lysosome-mediated degradation of NAMPT by measuring blockage of LC3 at 1 uM pretreated with probe N-(1-(3-benzyl-7-chloro-4-oxo-3,4-dihydroquinazolin-2-yl)-2-methylpropyl)-N-(3-(3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)thioureido)propyl)-4-methylbenzamide followed by addition of compound measured after 48 hrs by western blot analysis | Homo sapiens | 1 | ALA5154920 | cell-based format | Scientific Literature |