Protection against BoNT/A induced toxicity in mouse model assessed as survival rate at 2.5 mg/kg, ip preincubated with BoNT/A for 30 mins followed by administration into mouse and measured after 12 hrs (Rvb = 70%)
Protection against BoNT/A induced toxicity in mouse model assessed as survival rate at 2.5 mg/kg, ip preincubated with BoNT/A for 30 mins followed by administration into mouse and measured after 24 hrs relative to control
Irreversible inhibition of Clostridium botulinum BoNT/A light chain (1 to 424 residues) using SNAPtide as substrate at 1.5 uM preincubated for 2 hrs followed by substrate addition and measured after 1.5 hrs by fluorescence based analysis
Inhibition of Clostridium botulinum BoNT/A light chain (1 to 424 residues) using SNAPtide as substrate at 1.5 uM preincubated with ((4R,7S,10S,13S,16S,19S,22R)-13-((1H-indol-3-yl)methyl)-7-(4-aminobutyl)-19-(2-aminoethyl)-16-(3-guanidinopropyl)-10-((R)-1-hydroxyethyl)-6,9,12,15,18,21-hexaoxo-22-(3-(4-(3-oxobenzo[d][1,2]selenazol-2(3H)-yl)phenyl)propanamido)-1,2-dithia-5,8,11,14,17,20-hexaazacyclotricosane-4-carbonyl)-L-leucine for 2 hrs followed by substrate and compound addition and measured after 1.5 hrs by fluorescence based analysis