# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5123051 | B | Inhibition of recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3)pLysS cells/Nrf2 (unknown origin) protein-protein interaction using FITC-LDEETGEFL-NH2 as fluorescent substrate at 50 uM preincubated with compound for 30 mins followed by substrate addition and measured after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 35 | ALA5120835 | cell-based format | Scientific Literature | |
2. | ALA5123052 | B | Inhibition of recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3)pLysS cells/Nrf2 (unknown origin) protein-protein interaction using FITC-LDEETGEFL-NH2 as fluorescent substrate at 5 uM preincubated with compound for 30 mins followed by substrate addition and measured after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 35 | ALA5120835 | cell-based format | Scientific Literature | |
3. | ALA5123053 | B | Inhibition of recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3)pLysS cells/Nrf2 (unknown origin) protein-protein interaction using FITC-LDEETGEFL-NH2 as fluorescent substrate at 0.5 uM preincubated with compound for 30 mins followed by substrate addition and measured after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 35 | ALA5120835 | cell-based format | Scientific Literature | |
4. | ALA5123054 | B | Inhibition of recombinant human Keap1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3)pLysS cells/Nrf2 (unknown origin) protein-protein interaction using FITC-LDEETGEFL-NH2 as fluorescent substrate preincubated with compound for 30 mins followed by substrate addition and measured after 1 hr by fluorescence polarization assay relative to control | Homo sapiens | 35 | ALA5120835 | cell-based format | Scientific Literature | |
5. | ALA5167215 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase in GstP expression at 50 uM incubated for 16 hrs by RT-PCR analysis | Mus musculus | 1 | ALA5154913 | cell-based format | Scientific Literature | |
6. | ALA5167218 | B | Inhibition of Keap1-Nrf2 (unknown origin) protein-protein interaction expressed in Escherichia coli BL21 codon plus cells using FITC-beta-DEETGEF-OH as substrate incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 18 | ALA5154913 | cell-based format | Scientific Literature | |
7. | ALA5167219 | B | Binding affinity to Keap1 kelch domain (unknown origin) expressed in Escherichia coli BL21 codon plus cells assessed as inflection temperature at 10 uM incubated for 30 mins by differential scanning fluorimetry (Rvb = 61.3 +/- 0.1 degreeC) | Homo sapiens | 16 | ALA5154913 | cell-based format | Scientific Literature | |
8. | ALA5167220 | B | Binding affinity to Keap1 (unknown origin) expressed in Escherichia coli BL21 codon plus cells by isothermal titration calorimetry | Homo sapiens | 6 | ALA5154913 | cell-based format | Scientific Literature | |
9. | ALA5167221 | B | Activation of Nrf2 transcriptional activity in mouse Hepa1c1c7 cells assessed as fold induction in NQO1 activity at 10 uM after 24 hrs | Mus musculus | 15 | ALA5154913 | cell-based format | Scientific Literature | |
10. | ALA5167222 | B | Activation of Nrf2 transcriptional activity in mouse Hepa1c1c7 cells assessed as increase in NQO1 activity measured after 24 hrs | Mus musculus | 6 | ALA5154913 | cell-based format | Scientific Literature | |
11. | ALA5167225 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase in NQO1 expression at 50 uM incubated for 16 hrs by RT-PCR analysis | Mus musculus | 1 | ALA5154913 | cell-based format | Scientific Literature | |
12. | ALA5167226 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase in Gclc expression at 50 uM incubated for 16 hrs by RT-PCR analysis | Mus musculus | 1 | ALA5154913 | cell-based format | Scientific Literature | |
13. | ALA5167227 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase in Gclm expression at 50 uM incubated for 16 hrs by RT-PCR analysis | Mus musculus | 1 | ALA5154913 | cell-based format | Scientific Literature | |
14. | ALA5167228 | B | Activation of Nrf2 in mouse Hepa1c1c7 cells assessed as increase in Hmox1 expression at 50 uM incubated for 16 hrs by RT-PCR analysis | Mus musculus | 1 | ALA5154913 | cell-based format | Scientific Literature | |
15. | ALA5167229 | B | Binding affinity to Keap1 in human HL-60 cells assessed as increase in thermal stability at 50 uM incubated for 1 hr by cellular thermal shift assay | Homo sapiens | 1 | ALA5154913 | cell-based format | Scientific Literature | |
16. | ALA5167230 | P | Aqueous solubility of the compound at pH 7.4 | 1 | ALA5154913 | small-molecule physicochemical format | Scientific Literature | ||
17. | ALA5167241 | T | Cytotoxicity against human ARPE-19 cells assessed as reduction in cell viability upto 100 uM incubated for 96 hrs by MTT assay relative to control | Homo sapiens | 1 | ALA5154913 | cell-based format | Scientific Literature |