# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5108303 | B | Inhibition of N-terminal His-tagged recombinant human PARP4 brct-catalytic domain (369 to 573 residues) expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 3 | ALA5107944 | assay format | Scientific Literature | |
2. | ALA5108306 | B | Inhibition of N-terminal His-tagged full length human PARP3 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
3. | ALA5108307 | B | Inhibition of N-terminal His-tagged recombinant human PARP5 catalytic domain expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
4. | ALA5108308 | B | Inhibition of N-terminal His-tagged full length human PARP6 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
5. | ALA5108309 | B | Inhibition of N-terminal His-tagged full length human PARP7 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
6. | ALA5108310 | B | Inhibition of N-terminal His-tagged full length human PARP8 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
7. | ALA5108311 | B | Inhibition of N-terminal His-tagged human PARP10 catalytic domain expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
8. | ALA5108312 | B | Inhibition of N-terminal His-tagged full length human PARP11 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
9. | ALA5108313 | B | Inhibition of N-terminal His-tagged human PARP12 catalytic domain expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
10. | ALA5108314 | B | Inhibition of N-terminal His-tagged human PARP14 wwe-catalytic domain expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
11. | ALA5108316 | B | Inhibition of N-terminal His-tagged human PARP16 transmembrane deletion mutant expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 6 | ALA5107944 | assay format | Scientific Literature | |
12. | ALA5108317 | B | Inhibition of N-terminal His-tagged full length human PARP2 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 5 | ALA5107944 | assay format | Scientific Literature | |
13. | ALA5108318 | B | Inhibition of N-terminal His-tagged full length human PARP1 expressed in Escherichia coli BL21 (DE3) at 10 uM preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 4 | ALA5107944 | assay format | Scientific Literature | |
14. | ALA5108319 | B | Inhibition of N-terminal His-tagged human PARP15 catalytic domain expressed in Escherichia coli BL21 (DE3) preincubated with 6-a-NAD+ for 5 to 10 mins followed by enzyme addition and measured after 60 mins using NAD+ as substrate byHRP based chemiluminescence assay | Homo sapiens | 1 | ALA5107944 | assay format | Scientific Literature |