#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5140066	B	Inhibition of NLRP3 inflammasome in LPS-primed mouse J774.A1 cells assessed as reduction in IL-1beta secretion preincubated for 30 mins followed by ATP addition and measured after 30 mins by ELISA	Mus musculus	29	ALA5137033	cell-based format	Scientific Literature
2.	ALA5140067	T	Cytotoxicity against mouse J774.A1 cells assessed as cell viability at 10 uM incubated for 4 hrs by MTT assay relative to control	Mus musculus	29	ALA5137033	cell-based format	Scientific Literature
3.	ALA5140068	T	Cytotoxicity against HEK293 cells assessed as cell viability at 10 uM incubated for 4 hrs by MTT assay relative to control	Homo sapiens	29	ALA5137033	cell-based format	Scientific Literature
4.	ALA5140069	B	Inhibition of NLRC4 inflammasome in LPS-primed mouse J774.A1 cells assessed as IL-1beta secretion at 10 uM preincubated for 1 hr followed by flagellin addition and measured after 6 hrs by ELISA relative to control	Mus musculus	15	ALA5137033	cell-based format	Scientific Literature
5.	ALA5140070	B	Inhibition of AIM2 inflammasome in LPS-primed mouse J774.A1 cells assessed as IL-1beta secretion at 10 uM preincubated for 1 hr followed by poly(dA:dT) addition and measured after 4 hrs by ELISA relative to control	Mus musculus	15	ALA5137033	cell-based format	Scientific Literature
6.	ALA5140071	B	Inhibition of NLRC4 inflammasome in LPS-primed mouse J774.A1 cells assessed as reduction in IL-1beta secretion preincubated for 1 hr followed by flagellin addition and measured after 6 hrs by ELISA	Mus musculus	3	ALA5137033	cell-based format	Scientific Literature
7.	ALA5140072	B	Inhibition of AIM2 inflammasome in LPS-primed mouse J774.A1 cells assessed as reduction in IL-1beta secretion preincubated for 1 hr followed by poly(dA:dT) addition and measured after 4 hrs by ELISA	Mus musculus	3	ALA5137033	cell-based format	Scientific Literature
8.	ALA5140073	F	Inhibition of IL-6 production in LPS-primed mouse J774.A1 cells at 0.3 to 30 uM pretreated with compounds for 30 mins followed by LPS addition and measured after 16 hrs by ELISA	Mus musculus	1	ALA5137033	cell-based format	Scientific Literature
9.	ALA5140074	F	Inhibition of TNF-alpha production in LPS-primed mouse J774.A1 cells at 0.3 to 30 uM pretreated with compounds for 30 mins followed by LPS addition and measured after 16 hrs by ELISA	Mus musculus	1	ALA5137033	cell-based format	Scientific Literature
10.	ALA5140075	B	Inhibition of NLRP3 inflammasome in LPS-primed mouse BMDM cells assessed as reduction in IL-1beta secretion preincubated for 30 mins followed by ATP addition and measured after 30 mins by ELISA	Mus musculus	1	ALA5137033	cell-based format	Scientific Literature
11.	ALA5140076	B	Binding affinity to human recombinant full length NLRP3 transfected in HEK293F cells assessed as dissociation constant incubated for 40 mins by microscale thermophoresis analysis	Homo sapiens	1	ALA5137033	cell-based format	Scientific Literature
12.	ALA5140077	F	Inhibition of IL-1beta production in C57BL/6 mouse assessed as reduction in IL-1beta level in serum at 10 mg/kg, ip pretreated with compound followed by LPS addition and measured after 2.5 hrs by ELISA	Mus musculus	1	ALA5137033	organism-based format	Scientific Literature
13.	ALA5140078	F	Inhibition of TNF-alpha production in C57BL/6 mouse assessed as reduction in TNF-alpha level in serum at 10 mg/kg, ip pretreated with compound followed by LPS addition and measured after 2.5 hrs by ELISA	Mus musculus	1	ALA5137033	organism-based format	Scientific Literature