# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5135900 | T | Cytotoxicity against HEL 299 cells assessed as reduction in cell viability measured after 1 to 2 hrs by MTS colorimetric assay | Homo sapiens | 14 | ALA5131518 | cell-based format | Scientific Literature | |
2. | ALA5135901 | F | Antiviral activity against SARS-CoV-2 infected in eGFP expressing African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 4 days post infection by laser scanning microscopic analysis | Severe acute respiratory syndrome coronavirus 2 | 42 | ALA5131518 | organism-based format | Scientific Literature | |
3. | ALA5135902 | T | Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 1 to 2 hrs by MTS colorimetric assay | Chlorocebus sabaeus | 29 | ALA5131518 | cell-based format | Scientific Literature | |
4. | ALA5135903 | T | Selectivity index, ratio of CC50 for cytotoxicity against African green monkey Vero E6 cells to EC50 for antiviral activity against SARS-CoV-2 infected in eGFP expressing African green monkey Vero E6 cells | 18 | ALA5131518 | cell-based format | Scientific Literature | ||
5. | ALA5135904 | F | Antiviral activity against HCoV-229E infected in HEL 299 cells assessed as reduction in virus-induced cytopathic effect preincubated with compound followed by viral infection and measured after 7 days by MTS cell viability assay | Human coronavirus 229E | 14 | ALA5131518 | organism-based format | Scientific Literature | |
6. | ALA5135905 | T | Selectivity index, ratio of CC50 for cytotoxicity against HEL 299 cells to EC50 for antiviral activity against HCoV-229E infected in HEL 299 cells | 14 | ALA5131518 | cell-based format | Scientific Literature | ||
7. | ALA5135906 | F | Antiviral activity against HCoV-OC43 infected in HEL 299 cells assessed as reduction in virus-induced cytopathic effect preincubated with compound followed by viral infection and measured after 7 days by MTS cell viability assay | Human coronavirus OC43 | 14 | ALA5131518 | cell-based format | Scientific Literature | |
8. | ALA5135907 | T | Selectivity index, ratio of CC50 for cytotoxicity against HEL 299 cells to EC50 for antiviral activity against HCoV-OC43 infected in HEL 299 cells | 14 | ALA5131518 | cell-based format | Scientific Literature | ||
9. | ALA5135908 | B | Inhibition of SARS-CoV-2 RdRp expressed in Escherichia coli BL21 DE3 co-expressing nsp13 incubated for 1 hr by fluorescent based assay | Severe acute respiratory syndrome coronavirus 2 | 4 | ALA5131518 | assay format | Scientific Literature | |
10. | ALA5135909 | B | Inhibition of SARS-CoV-2 RdRp expressed in Escherichia coli BL21 DE3 co-expressing nsp13 at 30 uM incubated for 1 hr by fluorescent bases assay | Severe acute respiratory syndrome coronavirus 2 | 4 | ALA5131518 | assay format | Scientific Literature | |
11. | ALA5135910 | B | Inhibition of SARS-CoV-2 nsp13 helicase-associated activity using 5'- AGT CTT CTC CTG GTG CTC GAA CAG TGA CCy3-3', 5'- BHQ-2-GTC ACT GTT CGA GCA CCA CCT CTT CTG A-3' DNA as substrate preincubated for 10 mins followed by substrate addition and measured after 15 mins in the presence of ATP by fluorescent-based helicase unwind assay | Severe acute respiratory syndrome coronavirus 2 | 5 | ALA5131518 | single protein format | Scientific Literature | |
12. | ALA5135911 | B | Inhibition of SARS-CoV-2 nsp13 helicase-associated activity using 5'- AGT CTT CTC CTG GTG CTC GAA CAG TGA CCy3-3', 5'- BHQ-2-GTC ACT GTT CGA GCA CCA CCT CTT CTG A-3' DNA as substrate at highest concentration of compound preincubated for 10 mins followed by substrate addition and measured after 15 mins in the presence of ATP by fluorescent-based helicase unwind assay | Severe acute respiratory syndrome coronavirus 2 | 2 | ALA5131518 | single protein format | Scientific Literature | |
13. | ALA5135912 | B | Inhibition of SARS-CoV-2 nsp13 ATPase-associated activity using ATP as substrate incubated for 30 mins by Biomol Green Reagent based assay | Severe acute respiratory syndrome coronavirus 2 | 5 | ALA5131518 | single protein format | Scientific Literature | |
14. | ALA5135913 | B | Inhibition of SARS-CoV-2 nsp13 ATPase-associated activity using ATP as substrate at highest concentration of compound incubated for 30 mins by Biomol Green Reagent based assay | Severe acute respiratory syndrome coronavirus 2 | 5 | ALA5131518 | single protein format | Scientific Literature | |
15. | ALA5135915 | F | Inhibition of autophagy in African green monkey Vero E6 cells assessed as accumulation of LC3 puncta in cytoplasm at 10 uM measured after 4 hrs by DAPI staining based immunofluorescent confocal microscopic analysis | Chlorocebus sabaeus | 5 | ALA5131518 | cell-based format | Scientific Literature |