#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5135900	T	Cytotoxicity against HEL 299 cells assessed as reduction in cell viability measured after 1 to 2 hrs by MTS colorimetric assay	Homo sapiens	14		cell-based format	Scientific Literature
2.	ALA5135901	F	Antiviral activity against SARS-CoV-2 infected in eGFP expressing African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 4 days post infection by laser scanning microscopic analysis	Severe acute respiratory syndrome coronavirus 2	42		organism-based format	Scientific Literature
3.	ALA5135902	T	Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 1 to 2 hrs by MTS colorimetric assay	Chlorocebus sabaeus	29		cell-based format	Scientific Literature
4.	ALA5135903	T	Selectivity index, ratio of CC50 for cytotoxicity against African green monkey Vero E6 cells to EC50 for antiviral activity against SARS-CoV-2 infected in eGFP expressing African green monkey Vero E6 cells		18		cell-based format	Scientific Literature
5.	ALA5135904	F	Antiviral activity against HCoV-229E infected in HEL 299 cells assessed as reduction in virus-induced cytopathic effect preincubated with compound followed by viral infection and measured after 7 days by MTS cell viability assay	Human coronavirus 229E	14		organism-based format	Scientific Literature
6.	ALA5135905	T	Selectivity index, ratio of CC50 for cytotoxicity against HEL 299 cells to EC50 for antiviral activity against HCoV-229E infected in HEL 299 cells		14		cell-based format	Scientific Literature
7.	ALA5135906	F	Antiviral activity against HCoV-OC43 infected in HEL 299 cells assessed as reduction in virus-induced cytopathic effect preincubated with compound followed by viral infection and measured after 7 days by MTS cell viability assay	Human coronavirus OC43	14		cell-based format	Scientific Literature
8.	ALA5135907	T	Selectivity index, ratio of CC50 for cytotoxicity against HEL 299 cells to EC50 for antiviral activity against HCoV-OC43 infected in HEL 299 cells		14		cell-based format	Scientific Literature
9.	ALA5135908	B	Inhibition of SARS-CoV-2 RdRp expressed in Escherichia coli BL21 DE3 co-expressing nsp13 incubated for 1 hr by fluorescent based assay	Severe acute respiratory syndrome coronavirus 2	4		assay format	Scientific Literature
10.	ALA5135909	B	Inhibition of SARS-CoV-2 RdRp expressed in Escherichia coli BL21 DE3 co-expressing nsp13 at 30 uM incubated for 1 hr by fluorescent bases assay	Severe acute respiratory syndrome coronavirus 2	4		assay format	Scientific Literature
11.	ALA5135910	B	Inhibition of SARS-CoV-2 nsp13 helicase-associated activity using 5'- AGT CTT CTC CTG GTG CTC GAA CAG TGA CCy3-3', 5'- BHQ-2-GTC ACT GTT CGA GCA CCA CCT CTT CTG A-3' DNA as substrate preincubated for 10 mins followed by substrate addition and measured after 15 mins in the presence of ATP by fluorescent-based helicase unwind assay	Severe acute respiratory syndrome coronavirus 2	5		single protein format	Scientific Literature
12.	ALA5135911	B	Inhibition of SARS-CoV-2 nsp13 helicase-associated activity using 5'- AGT CTT CTC CTG GTG CTC GAA CAG TGA CCy3-3', 5'- BHQ-2-GTC ACT GTT CGA GCA CCA CCT CTT CTG A-3' DNA as substrate at highest concentration of compound preincubated for 10 mins followed by substrate addition and measured after 15 mins in the presence of ATP by fluorescent-based helicase unwind assay	Severe acute respiratory syndrome coronavirus 2	2		single protein format	Scientific Literature
13.	ALA5135912	B	Inhibition of SARS-CoV-2 nsp13 ATPase-associated activity using ATP as substrate incubated for 30 mins by Biomol Green Reagent based assay	Severe acute respiratory syndrome coronavirus 2	5		single protein format	Scientific Literature
14.	ALA5135913	B	Inhibition of SARS-CoV-2 nsp13 ATPase-associated activity using ATP as substrate at highest concentration of compound incubated for 30 mins by Biomol Green Reagent based assay	Severe acute respiratory syndrome coronavirus 2	5		single protein format	Scientific Literature
15.	ALA5135915	F	Inhibition of autophagy in African green monkey Vero E6 cells assessed as accumulation of LC3 puncta in cytoplasm at 10 uM measured after 4 hrs by DAPI staining based immunofluorescent confocal microscopic analysis	Chlorocebus sabaeus	5		cell-based format	Scientific Literature