| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5132491 | B | Inhibition of FTO (unknown origin) demethylation activity by HPLC assay | Homo sapiens | 3 | ALA5131390 | single protein format | Scientific Literature | |
| 2. | ALA5216090 | B | Inhibition of human KDM6B preincubated for 15 mins followed by substrate addition and measured after 5 mins using H3(21-44)K27Me3-GK-biotin peptide as substrate by AlphaScreen-based assay | Homo sapiens | 12 | ALA5214918 | single protein format | Scientific Literature | |
| 3. | ALA5216102 | B | Inhibition of recombinant full length N-terminal hexahistidine-tagged human FTO demethylation activity expressed in Escherichia coli BL21 (DE3) incubated for 30 mins using 5-mer ssRNA oligonucleotide [GG(m6A)CU] as substrate by LCMS-based assay | Homo sapiens | 12 | ALA5214918 | assay format | Scientific Literature | |
| 4. | ALA5216104 | B | Inhibition of recombinant N-terminal hexahistidine-tagged human PDH2 (181 to 426 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins followed by substrate addition and measured after 15 mins using CODD peptide DLDLEMLAPYIPMDDDFQL as substrate by SPE-MS-based assay | Homo sapiens | 12 | ALA5214918 | assay format | Scientific Literature | |
| 5. | ALA5216106 | B | Inhibition of human KDM3A preincubated for 15 mins followed by substrate addition and measured after 5 mins using H3(1-21)K9Me2-GGK-Biotin peptide as substrate by AlphaScreen-based assay | Homo sapiens | 12 | ALA5214918 | single protein format | Scientific Literature | |
| 6. | ALA5216107 | B | Inhibition of human KDM4C preincubated for 15 mins followed by substrate addition and measured after 15 mins using H3(1-21)K9Me3-GGK-Biotin peptide as substrate by AlphaScreen-based assay | Homo sapiens | 12 | ALA5214918 | single protein format | Scientific Literature | |
| 7. | ALA5216108 | B | Inhibition of human KDM5B preincubated for 15 mins followed by substrate addition and measured after 20 mins using H3(1-21)K4Me3-GGK-Biotin peptide as substrate by AlphaScreen-based assay | Homo sapiens | 12 | ALA5214918 | single protein format | Scientific Literature | |
| 8. | ALA5216109 | B | Inhibition of human KDM2A preincubated for 15 mins followed by substrate addition and measured after 30 mins using Biotin-H3(28-48)K36Me2 peptide as substrate by AlphaScreen-based assay | Homo sapiens | 12 | ALA5214918 | single protein format | Scientific Literature | |
| 9. | ALA5229116 | B | Inhibition of Escherichia coli AlkB using 15-mer m1A-ssDNA as substrate incubated for 10 mins by HPLC analysis | Escherichia coli | 3 | ALA5226352 | single protein format | Scientific Literature | |
| 10. | ALA5229117 | B | Inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells using 3-methylthymidine as substrate incubated for 1 hr by HPLC analysis | Homo sapiens | 8 | ALA5226352 | cell-based format | Scientific Literature | |
| 11. | ALA5229118 | B | Inhibition of human ALKBH2 (56 to 258 residues) expressed in Escherichia coli BL21 (DE3) cells using 15-mer m1A-ssDNA as substrate incubated for 10 mins by HPLC analysis | Homo sapiens | 3 | ALA5226352 | cell-based format | Scientific Literature | |
| 12. | ALA5229119 | B | Inhibition of N-terminal His-tagged human ALKBH3 (1 to 286 residues) using m6A-ssDNA as substrate incubated for 30 mins by MALDI-TOF-MS analysis | Homo sapiens | 3 | ALA5226352 | single protein format | Scientific Literature | |
| 13. | ALA5229120 | B | Inhibition of human ALKBH5 expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells using 5-mer m6A-ssRNA as substrate incubated for 30 mins by HPLC analysis | Homo sapiens | 2 | ALA5226352 | cell-based format | Scientific Literature | |
| 14. | ALA5229139 | B | Inhibition of C-terminal MYC/DDK tagged full length human PHD2 using 19 mer peptide substrate DLDLEMLAPYIPMDDDFQL incubated for 30 mins by MALDI-based assay | Homo sapiens | 1 | ALA5226352 | single protein format | Scientific Literature | |
| 15. | ALA5229140 | B | Inhibition of human KDM4A expressed in Escherichia coli BL21 (DE3) rosetta T1R cells using ARK(me3)STGGK as substrate incubated for 30 mins by MALDI-TOF-MS analysis | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature | |
| 16. | ALA5229141 | B | Selectivity index, ratio of IC50 for inhibition of C-terminal MYC/DDK tagged full length human PHD2 to IC50 for inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature | |
| 17. | ALA5229142 | B | Selectivity index, ratio of IC50 for inhibition of human KDM4A expressed in Escherichia coli BL21 (DE3) rosetta T1R cells to IC50 for inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature | |
| 18. | ALA5229143 | B | Selectivity index, ratio of IC50 for inhibition of human ALKBH2 (56 to 258 residues) expressed in Escherichia coli BL21 (DE3) cells to IC50 for inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature | |
| 19. | ALA5229144 | B | Selectivity index, ratio of IC50 for inhibition of N-terminal His-tagged human ALKBH3 (1 to 286 residues) to IC50 for inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature | |
| 20. | ALA5229145 | B | Selectivity index, ratio of IC50 for inhibition of human ALKBH5 expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells to IC50 for inhibition of human full length FTO expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells | Homo sapiens | 1 | ALA5226352 | cell-based format | Scientific Literature |
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