# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5129610 | Binding | Inhibition of NFATC activity in HEK293T cells at 5 to 50 uM preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs by luciferase reporter assay | Homo sapiens | 11 | ALA5126596 | cell-based format | Scientific Literature | |
2. | ALA5129612 | Binding | Inhibition of NFATC activity in HEK293T cells preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs by luciferase reporter assay | Homo sapiens | 7 | ALA5126596 | cell-based format | Scientific Literature | |
3. | ALA5129613 | Binding | Displacement of carboxyfluorescein labelled NFATc2 derived PRIEIT peptide from human CNAalpha (2 to 347 residues) at 10 to 100 uM preincubated for 15 mins followed by CF-PRIEIT addition and measured after 15 mins by fluorescence polarization assay | Homo sapiens | 9 | ALA5126596 | single protein format | Scientific Literature | |
4. | ALA5129614 | Binding | Inhibition of NFATC activity in human PBMC cells assessed as reduction of IL2 gene expression preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs by RT-PCR analysis | Homo sapiens | 3 | ALA5126596 | cell-based format | Scientific Literature | |
5. | ALA5129615 | Binding | Inhibition of NFATC activity in human PBMC cells assessed as reduction of IFN gamma gene expression preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs byRT-PCR analysis | Homo sapiens | 3 | ALA5126596 | cell-based format | Scientific Literature | |
6. | ALA5129616 | Binding | Inhibition of NFATC activity in human CD4+ T cells assessed as reduction of IFN gamma secretion preincubated for 2 hrs followed by anti-CD3/CD28 stimulation and measured after 48 hrs by ELISA | Homo sapiens | 3 | ALA5126596 | cell-based format | Scientific Literature | |
7. | ALA5129617 | Binding | Inhibition of NFATC activity in human CD4+ T cells assessed as reduction of IL-2 secretion preincubated for 2 hrs followed by anti-CD3/CD28 stimulation and measured after 48 hrs by ELISA | Homo sapiens | 3 | ALA5126596 | cell-based format | Scientific Literature | |
8. | ALA5129618 | Functional | Immunosuppressive activity in human CD4+ve Th cells assessed as inhibition of T cell proliferation measured after 72 hrs by [3H]-thymidine incorporation based scintillation counter method | Homo sapiens | 3 | ALA5126596 | cell-based format | Scientific Literature | |
9. | ALA5129619 | Toxicity | Toxicity in BALB/c mouse assessed as clinical signs of abnormal constitution at 50 mg/kg, ip administered for 5 days and measured on day 7 | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
10. | ALA5129620 | Toxicity | Toxicity in BALB/c mouse assessed as aberrant behavior at 50 mg/kg, ip administered for 5 days and measured on day 7 | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
11. | ALA5129621 | Toxicity | Toxicity in BALB/c mouse assessed as loss of body weight at 50 mg/kg, ip administered for 5 days and measured on day 7 | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
12. | ALA5129622 | Toxicity | Toxicity in BALB/c mouse xenografted with mouse 4T1 cells assessed as effect on body weight at 50 mg/kg, ip administered daily for 5 days | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
13. | ALA5129624 | Toxicity | Toxicity in BALB/c mouse xenografted with mouse 4T1 cells assessed as effect on health conditions at 50 mg/kg, ip administered daily for 5 days | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
14. | ALA5129627 | Functional | Antitumor activity against mouse 4T1 cells xenografted in BALB/c mouse assessed as decrease in tumor weight at 5 mg/kg, ip measured after 15 days | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature | |
15. | ALA5129629 | Functional | Anti-angiogenesis activity against mouse 4T1 cells xenografted in BALB/c mouse at 50 mg/kg, ip measured after 15 days by anti-CD31 staining based immunohistochemistry analysis | Mus musculus | 2 | ALA5126596 | organism-based format | Scientific Literature |