| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5098726 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 2. | ALA5098727 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
| 3. | ALA5098728 | Binding | Antagonist activity at N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
| 4. | ALA5098729 | Binding | Antagonist activity at N-terminal His-tagged cIAP1-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
| 5. | ALA5098730 | Binding | Antagonist activity at N-terminal His-tagged cIAP2-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
| 6. | ALA5098731 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 7. | ALA5098732 | Binding | Antagonist activity at N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 8. | ALA5098733 | Binding | Antagonist activity at N-terminal His-tagged cIAP1-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 9. | ALA5098734 | Binding | Antagonist activity at N-terminal His-tagged cIAP2-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 10. | ALA5098735 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues)(unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:2 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 11. | ALA5098736 | Binding | Antagonist activity at N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues)(unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:2 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 12. | ALA5098737 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:10 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 13. | ALA5098738 | Binding | Binding affinity to N-terminal His-tagged ML-IAP BIR domain K135A mutant (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:10 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 14. | ALA5098739 | Binding | Binding affinity to N-terminal His-tagged ML-IAP BIR domain K121A mutant (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 at protein:compound concentration ratio of 1:10 incubated for 2 hrs by SDS-PAGE analysis | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 15. | ALA5098740 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as inhibition constant using AVPIAQKSEK-biotinylated peptide as substrate incubated upto 40 mins by DELFIA assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 16. | ALA5098741 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as inactivation constant using AVPIAQKSEK-biotinylated peptide as substrate incubated upto 40 mins by DELFIA assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 17. | ALA5098742 | Binding | Binding affinity to N-terminal His-tagged ML-IAP K135A mutant (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
| 18. | ALA5098743 | Binding | Binding affinity to ML-IAP in human SK-MEL-28 cells assessed as change in melting temperature at 10 uM incubated for 3 hrs followed by heating at 37 to 70 degree C for 3 mins by CETSA analysis (Rvb = 50 degreeC) | Homo sapiens | 3 | ALA5096205 | cell-based format | Scientific Literature | |
| 19. | ALA5098744 | Binding | Binding affinity to N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as non-covalent stabilization by measuring change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature | |
| 20. | ALA5098745 | Binding | Binding affinity to N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as covalent adduct formation by measuring change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 1 | ALA5096205 | assay format | Scientific Literature |
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