# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5132099 | F | Agonist activity at wild type GPR88 in C57BL/6J mouse striatal membrane assessed as maximum efficacy incubated for 1 hr in presence of [35S]GTPgammaS by scintillation counting method relative to control relative to control | Mus musculus | 1 | ALA5131380 | cell membrane format | Scientific Literature | |
2. | ALA5132100 | F | Agonist activity at GPR88 (unknown origin) expressed in CHO cells coexpressing PPLS-HA assessed as reduction in forskolin-stimulated cAMP production and measured after 90 mins by Lance TR-FRET cAMP assay | Homo sapiens | 40 | ALA5131380 | cell-based format | Scientific Literature | |
3. | ALA5132101 | F | Agonist activity at GPR88 (unknown origin) expressed in CHO cells coexpressing PPLS-HA assessed as reduction in forskolin-stimulated cAMP production by measuring maximum efficacy and measured after 90 mins by Lance TR-FRET cAMP assay | Homo sapiens | 36 | ALA5131380 | cell-based format | Scientific Literature | |
4. | ALA5132102 | F | Agonist activity at wild type GPR88 in mouse striatal membrane assessed as maximum efficacy incubated for 1 hr in presence of [35S]GTPgammaS by scintillation counting method relative to control relative to control | Mus musculus | 1 | ALA5131380 | cell membrane format | Scientific Literature | |
5. | ALA5132103 | F | Agonist activity at wild type GPR88 expressed in mouse striatal membrane assessed as [35S] GTPgammaS binding incubated for 1 hr by liquid scintillation counting method | Mus musculus | 1 | ALA5131380 | cell membrane format | Scientific Literature | |
6. | ALA5132104 | F | Agonist activity at wild type GPR88 expressed in C57BL/6J mouse striatal membrane assessed as [35S] GTPgammaS binding incubated for 1 hr by liquid scintillation counting method | Mus musculus | 1 | ALA5131380 | cell membrane format | Scientific Literature | |
7. | ALA5132105 | F | Potency index, ratio of RTI-13951-33 EC50 to test compound EC50 for agonist activity at GPR88 (unknown origin) expressed in CHO cells coexpressing PPLS-HA assessed as reduction in forskolin-stimulated cAMP production and measured after 90 mins by Lance TR-FRET cAMP assay | Homo sapiens | 1 | ALA5131380 | cell-based format | Scientific Literature | |
8. | ALA5132106 | A | Apparent permeability across apical to basolateral side in MDCK-MDR1 cells by LC-MS/MS analysis | Canis lupus familiaris | 4 | ALA5131380 | cell-based format | Scientific Literature | |
9. | ALA5132107 | A | Apparent permeability across basolateral to apical side in MDCK-MDR1 cells by LC-MS/MS analysis | Canis lupus familiaris | 4 | ALA5131380 | cell-based format | Scientific Literature | |
10. | ALA5132108 | A | Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in MDCK-MDR1 cells by LC-MS/MS analysis | Canis lupus familiaris | 4 | ALA5131380 | cell-based format | Scientific Literature | |
11. | ALA5132109 | A | Cmax in C57BL/6NCrl mouse plasma at 20 mg/kg, ip measured after 2 hrs by LC-MS/MS analysis | Mus musculus | 1 | ALA5131380 | organism-based format | Scientific Literature | |
12. | ALA5132110 | A | Metabolic stability in C57BL/6NCrl mouse plasma assessed as half life at 20 mg/kg, ip by LC-MS/MS analysis | Mus musculus | 1 | ALA5131380 | organism-based format | Scientific Literature | |
13. | ALA5132111 | A | Clearance in C57BL/6NCrl mouse plasma at 20 mg/kg, ip by LC-MS/MS analysis | Mus musculus | 1 | ALA5131380 | organism-based format | Scientific Literature | |
14. | ALA5132112 | A | Cmax in C57BL/6NCrl mouse brain at 20 mg/kg, ip measured after 2 hrs by LC-MS/MS analysis | Mus musculus | 1 | ALA5131380 | organism-based format | Scientific Literature | |
15. | ALA5132113 | A | Ratio of drug level in brain to plasma in C57BL/6NCrl mouse at 20 mg/kg, ip by LC-MS/MS analysis | Mus musculus | 1 | ALA5131380 | organism-based format | Scientific Literature | |
16. | ALA5132115 | F | Agonist activity at GPR88 in GPR88 knockout mouse striatal membrane assessed as [35S] GTPgammaS binding at 100 uM incubated for 1 hr by liquid scintillation counting method | Mus musculus | 1 | ALA5131380 | cell membrane format | Scientific Literature |