# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5142555 | B | Binding affinity to recombinant human active PKAC-alpha assessed as compound-protein adduct formation by measuring formation of three bands at 1.5 uM incubated for 1 hr by 5-TAMRA dye based immunostaining analysis | Homo sapiens | 1 | ALA5137094 | single protein format | Scientific Literature | |
2. | ALA5142556 | B | Binding affinity to recombinant human denatured PKAC-alpha assessed as compound-protein adduct formation by measuring formation of three bands at 1.5 uM incubated for 1 hr by 5-TAMRA dye based immunostaining analysis | Homo sapiens | 1 | ALA5137094 | single protein format | Scientific Literature | |
3. | ALA5142557 | B | Binding affinity to recombinant human spiked PKAC-alpha in human U2OS cell lysates assessed as covalent compound-protein adduct formation at 1.5 uM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
4. | ALA5142558 | B | Binding affinity to recombinant human spiked PKAC-alpha in human U2OS cell lysates assessed as covalent compound-protein adduct formation at 1.5 uM incubated for 1 hr in presence of PK inhibitor, staurosporin by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
5. | ALA5142559 | B | Binding affinity to recombinant human spiked PKAC-alpha in human U2OS cell lysates assessed as covalent compound-protein adduct formation at 1.5 uM incubated for 1 hr in presence of GSH by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
6. | ALA5142560 | B | Binding affinity to recombinant human spiked PKAC-alpha in human HeLa cells assessed as covalent compound-protein adduct formation by measuring multiple fluorescent band formation at 1.5 uM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | cell-based format | Scientific Literature | |
7. | ALA5142561 | B | Binding affinity to recombinant human spiked PKAC-alpha in human HeLa cells assessed as covalent compound-protein adduct formation by measuring multiple fluorescent band formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | cell-based format | Scientific Literature | |
8. | ALA5142562 | B | Binding affinity to recombinant human spiked PKAC-alpha in human PC-3 cell lysates assessed as covalent compound-protein adduct formation at 15 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
9. | ALA5142563 | B | Binding affinity to recombinant full length human spiked PKAC-beta in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
10. | ALA5142564 | B | Binding affinity to N-terminal 6His-tagged active recombinant human spiked Akt3 S472D mutant (117 to end residues) in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
11. | ALA5142565 | B | Binding affinity to N-terminal 6His-tagged recombinant human spiked MSK1 (2 to end residues) in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
12. | ALA5142566 | B | Binding affinity to N-terminal 6His-tagged active recombinant full length human spiked Aurora A in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
13. | ALA5142567 | B | Binding affinity to GST-tagged recombinant full length human ROCK-2 catalytic domain (1 to 552 residues) in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation at 150 nM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
14. | ALA5142568 | B | Binding affinity to recombinant full length human spiked PKAC-beta in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation by measuring remaining activity at 150 nM measured after 1 hr by PKC antibody immunostaining based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
15. | ALA5142569 | B | Binding affinity to N-terminal 6His-tagged active recombinant human spiked Akt3 S472D mutant (117 to end residues) in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation by measuring remaining activity at 150 nM incubated for 1 hr by Akt3 antibody immunostaining based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature | |
16. | ALA5142570 | B | Binding affinity to N-terminal 6His-tagged active recombinant full length human spiked Aurora A in human PC-3 cell lysates assessed as irreversible compound-protein adduct formation by measuring remaining activity at 150 nM incubated for 1 hr by Aurora A antibody immunostaining based Western blot analysis | Homo sapiens | 1 | ALA5137094 | subcellular format | Scientific Literature |