# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5166862 | B | Competitive inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate assessed as inhibition constant by Michaelis-Menten analysis | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
2. | ALA5166863 | A | Stability of the compound in pooled human plasma at 3 mM measured up to 24 hrs by UV/LC-MS assay | Homo sapiens | 2 | ALA5154905 | cell-free format | Scientific Literature | |
3. | ALA5166864 | A | Metabolic stability in human liver microsomes incubated for 1 hr in presence of NADPH regenerating system by UV/LC-MS analysis | Homo sapiens | 2 | ALA5154905 | microsome format | Scientific Literature | |
4. | ALA5166865 | P | Stability of the compound in MeOH solution at 500 uM measured up to 24 hrs by UV/LC-MS analysis | 2 | ALA5154905 | small-molecule physicochemical format | Scientific Literature | ||
5. | ALA5166868 | F | Antimigratory activity in human PDAC-2 cells assessed as reduction in wound closure at 36 uM measured after 20 hrs by scratch wound healing assay (Rvb = 70 +/- 3%) | Homo sapiens | 1 | ALA5154905 | cell-based format | Scientific Literature | |
6. | ALA5166869 | A | Apparent permeability of the compound in PBS buffer pH 7.4 assessed as membrane retention at 0.5 mM incubated for 5 hrs by PAMPA based UV/LC-MS analysis | 2 | ALA5154905 | assay format | Scientific Literature | ||
7. | ALA5166870 | B | Inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate incubated for 30 mins by microplate reader method | Homo sapiens | 14 | ALA5154905 | single protein format | Scientific Literature | |
8. | ALA5166871 | B | Inhibition of human recombinant FAAH using AMC arachidonoyl amide as substrate incubated for 30 mins by fluorescence based assay | Homo sapiens | 14 | ALA5154905 | single protein format | Scientific Literature | |
9. | ALA5166872 | B | Inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate incubated for 15 mins in presence of DTT by microplate reader analysis | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
10. | ALA5166873 | B | Reversible inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate preincubated for 30 to 60 mins followed by substrate addition and measured after 30 mins by microplate reader analysis | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
11. | ALA5166874 | B | Reversible inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate at 320 nM preincubated for 60 mins followed by 40X dilution with buffer and further incubated with substrate for 15 mins by microplate reader analysis | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
12. | ALA5166875 | B | Reversible inhibition of human recombinant MAGL using 4-nitrophenylacetate as substrate at 8 nM preincubated for 60 mins followed by 40X dilution with buffer and further incubated with substrate for 15 mins by microplate reader analysis | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
13. | ALA5166876 | B | Displacement of [3H]-CP55940 from human CB2 receptor incubated for 90 mins | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
14. | ALA5166877 | B | Displacement of [3H]-CP55940 from human CB1 receptor incubated for 90 mins | Homo sapiens | 1 | ALA5154905 | single protein format | Scientific Literature | |
15. | ALA5166878 | B | Inhibition of human recombinant MAGL in mouse brain membrane at 10 uM using TAMRA-FP serine hydrolase probe as substrate preincubated for 25 mins followed by substrate addition and measured after 25 mins SDS-PAGE analysis | Homo sapiens | 3 | ALA5154905 | cell membrane format | Scientific Literature | |
16. | ALA5166879 | B | Inhibition of FAAH in mouse brain membrane at 10 uM using TAMRA-FP serine hydrolase probe as substrate preincubated for 25 mins followed by substrate addition and measured after 25 mins SDS-PAGE analysis | Mus musculus | 3 | ALA5154905 | cell membrane format | Scientific Literature | |
17. | ALA5166880 | B | Inhibition of ABHD6 in mouse brain membrane at 10 uM using TAMRA-FP serine hydrolase probe as substrate preincubated for 25 mins followed by substrate addition and measured after 25 mins SDS-PAGE analysis | Mus musculus | 4 | ALA5154905 | cell membrane format | Scientific Literature | |
18. | ALA5166881 | B | Inhibition of ABHD12 in mouse brain membrane at 10 uM using TAMRA-FP serine hydrolase probe as substrate preincubated for 25 mins followed by substrate addition and measured after 25 mins SDS-PAGE analysis | Mus musculus | 3 | ALA5154905 | cell membrane format | Scientific Literature | |
19. | ALA5166882 | F | Antiproliferative activity against human PDAC-2 cells assessed as cell growth inhibition incubated for 72 hrs by sulforhodamine B assay | Homo sapiens | 1 | ALA5154905 | cell-based format | Scientific Literature | |
20. | ALA5166883 | F | Antiproliferative activity against human PDAC-3 cells assessed as cell growth inhibition incubated for 72 hrs by sulforhodamine B assay | Homo sapiens | 3 | ALA5154905 | cell-based format | Scientific Literature |