| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5142536 | B | Displacement of ARC-1063 fluorescent probe from recombinant human PKAC-alpha assessed as dissociation half life preincubated for 1 hr followed by photoluminescent probe addition by time-gated luminescence intensity based assay | Homo sapiens | 7 | ALA5137094 | single protein format | Scientific Literature | |
| 2. | ALA5142545 | B | Binding affinity to human recombinant PKAC-alpha assessed as reduction in TGLI signal at 10 uM pretreated with compound followed by ARC-1063 fluorescent probe addition by time-gated luminescence intensity based displacement assay | Homo sapiens | 4 | ALA5137094 | single protein format | Scientific Literature | |
| 3. | ALA5142546 | B | Binding affinity to human recombinant PKAC-alpha assessed as inactivation constant incubated for 1 hr by time-gated luminescence intensity based displacement assay | Homo sapiens | 4 | ALA5137094 | single protein format | Scientific Literature | |
| 4. | ALA5142547 | B | Binding affinity to human recombinant PKAC-alpha assessed as inactivation constant measured upto 60 mins by Western blot analysis | Homo sapiens | 4 | ALA5137094 | single protein format | Scientific Literature | |
| 5. | ALA5142548 | B | Covalent inhibition of human recombinant PKAC-alpha assessed as inhibition constant | Homo sapiens | 4 | ALA5137094 | single protein format | Scientific Literature | |
| 6. | ALA5142549 | B | Covalent inhibition of human recombinant PKAC-alpha assessed as ratio of Kinact/Ki | Homo sapiens | 4 | ALA5137094 | single protein format | Scientific Literature | |
| 7. | ALA5142550 | P | GSH reactivity in potassium phosphate buffer at pH 7.4 assessed as half life at 0.15 mM at 30 degreeC measured for 30 to 60 mins by HPLC-MS analysis | 1 | ALA5137094 | small-molecule physicochemical format | Scientific Literature | ||
| 8. | ALA5142551 | P | GSH reactivity in potassium phosphate buffer at pH 7.4 assessed as half life at 0.15 mM at 37 degreeC measured for 30 to 60 mins by HPLC-MS analysis | 2 | ALA5137094 | small-molecule physicochemical format | Scientific Literature | ||
| 9. | ALA5142552 | A | Drug degradation in phosphate buffer at pH 7.4 assessed as half life at 0.15 mM at 30 degreeC measured for 30 to 60 mins by HPLC-MS analysis | 1 | ALA5137094 | assay format | Scientific Literature | ||
| 10. | ALA5142553 | A | Drug degradation in phosphate buffer at pH 7.4 assessed as half life at 0.15 mM at 37 degreeC measured for 30 to 60 mins by HPLC-MS analysis | 1 | ALA5137094 | assay format | Scientific Literature | ||
| 11. | ALA5142554 | B | Binding affinity to human recombinant PKACalpha assessed as compound-protein adduct formation by measuring half life at 30 degreeC | Homo sapiens | 3 | ALA5137094 | single protein format | Scientific Literature | |
| 12. | ALA5142580 | B | Binding affinity to human recombinant PKAC-alpha in human HeLa cells at 5 uM by Western blot analysis | Homo sapiens | 1 | ALA5137094 | cell-based format | Scientific Literature | |
| 13. | ALA5142591 | B | Binding affinity to human recombinant PKAC-alpha at Cys199 residue assessed as compound-protein adduct formation by measuring mass difference at 3.5 uM incubated for 2 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA5137094 | single protein format | Scientific Literature | |
| 14. | ALA5142592 | B | Binding affinity to human recombinant PKAC-alpha at Cys343 residue assessed as compound-protein adduct formation by measuring mass difference at 3.5 uM incubated for 2 hrs by LC-MS/MS analysis | Homo sapiens | 1 | ALA5137094 | single protein format | Scientific Literature |
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