| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5138368 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as reduction in caspase 1 p20 level in cell supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 2. | ALA5138369 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced cleaved IL-1beta secretion in cell supernatant preincubated for 30 mins followed by ATP stimulation for 5 hrs by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 3. | ALA5138370 | Binding | Inhibition of AIM2 inflammasome activation in LPS-primed human THP-1 cells assessed as attenuation of ASC oligomerization at 0.1 to 10 uM preincubated for 30 mins followed by overnight stimulation with poly(dA:dT) by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 4. | ALA5138371 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 ASC knock out cells reconstituted with mouse ASC assessed as inhibition of IL-1beta release at 0.1 to 10 uM preincubated for 30 mins followed by nigericin stimulation and measured after 30 mins by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 5. | ALA5138372 | Binding | Inhibition of NLRP3 inflammasome in LPS-primed human THP-1 cells assessed as intracellular potassium level preincubated for 30 mins followed by nigericin stimulation and measured after 30 mins by Potassium turbidimetric assay | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 6. | ALA5138373 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as increase in JNK phosphorylation at 10 uM preincubated for 30 mins followed by nigericin stimulation and measured after 30 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 7. | ALA5138374 | Binding | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-stimulated PMA-differentiated human THP-1 macrophages assessed as reduction in pyroptosis pretreated with LPS followed by compound treatment for 30 mins then stimulation with nigericin and measured after 3 hrs by MTT assay | Homo sapiens | 68 | ALA5137000 | cell-based format | Scientific Literature | |
| 8. | ALA5138376 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as reduction in lactate dehydrogenase level pretreated for 30 mins followed by nigericin stimulation and measured after 60 min by LDH assay | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 9. | ALA5138377 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as reduction in IL-1beta release in supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 10. | ALA5138378 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as reduction in IL-18 release in supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 11. | ALA5138379 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as reduction in TNFalpha release in supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 12. | ALA5138380 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as cleavage of pro-IL-1beta in cell supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 13. | ALA5138381 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as cleavage of GSDMD in cell supernatant pretreated for 30 mins followed by nigericin stimulation and measured after 30 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 14. | ALA5138382 | Binding | Inhibition of NLRP3 inflammasome activation in human THP-1 cells assessed as LPS-induced pro-IL-1beta expression level preincubated with LPS for 4 hrs followed by compound treatment for 60 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 15. | ALA5138383 | Binding | Inhibition of NLRP3 inflammasome activation in human THP-1 cells assessed as LPS-induced NLRP3 expression level preincubated with LPS for 4 hrs followed by compound treatment for 60 mins by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 16. | ALA5138384 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced cleaved IL-1beta secretion preincubated for 30 mins followed by ATP stimulation for 5 hrs by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 17. | ALA5138385 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced cleaved caspase -1 secretion preincubated for 30 mins followed by ATP stimulation for 5 hrs by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 18. | ALA5138386 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced GSDMD cleavage preincubated for 30 mins followed by ATP stimulation for 5 hrs by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 19. | ALA5138387 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced cleaved IL-1beta secretion in cell supernatant preincubated for 30 mins followed by MSU stimulation for 4 hrs by ELISA | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature | |
| 20. | ALA5138388 | Binding | Inhibition of NLRP3 inflammasome activation in LPS-primed human THP-1 cells assessed as suppression of ATP-induced cleaved IL-1beta secretion preincubated for 30 mins followed by MSU stimulation for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA5137000 | cell-based format | Scientific Literature |
The page will load shortly, Thanks for your patience!