| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5123586 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 8 | ALA5120856 | cell-based format | Scientific Literature | |
| 2. | ALA5123587 | B | Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay | Mus musculus | 8 | ALA5120856 | cell-based format | Scientific Literature | |
| 3. | ALA5123590 | B | Inhibition of human recombinant LDHA using pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 10 | ALA5120856 | single protein format | Scientific Literature | |
| 4. | ALA5123591 | B | Inhibition of human recombinant LDHB using pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 9 | ALA5120856 | single protein format | Scientific Literature | |
| 5. | ALA5123594 | B | Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay | Homo sapiens | 4 | ALA5120856 | cell-based format | Scientific Literature | |
| 6. | ALA5123595 | B | Non-competitive mixed inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition constant using varying concentration of glycolate as substrate measured every 60 sec for 15 mins by Amplex red and horseradish peroxidase based fluorescence assay | Mus musculus | 4 | ALA5120856 | cell-based format | Scientific Literature | |
| 7. | ALA5123596 | B | Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 10 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay | Homo sapiens | 3 | ALA5120856 | cell-based format | Scientific Literature | |
| 8. | ALA5123597 | B | Reversible inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as enzyme recovery using glycolate as substrate at 100 times of IC50 concentration preincubated for 30 mins followed by substrate addition and measured every 60 sec for 15 mins by jump-dilution based Amplex red and horseradish peroxidase fluorescence assay | Homo sapiens | 3 | ALA5120856 | cell-based format | Scientific Literature | |
| 9. | ALA5123598 | B | Non-competitive inhibition of human recombinant LDHA assessed as inhibition constant using varying concentration of pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 4 | ALA5120856 | single protein format | Scientific Literature | |
| 10. | ALA5123600 | B | Non-competitive inhibition of human recombinant LDHB assessed as inhibition constant using varying concentration of pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 4 | ALA5120856 | single protein format | Scientific Literature | |
| 11. | ALA5123602 | B | Ratio of Ki for Non-competitive inhibition of human recombinant LDHB to Ki for non-competitive inhibition of human recombinant LDHA | Homo sapiens | 4 | ALA5120856 | assay format | Scientific Literature | |
| 12. | ALA5123604 | B | Non-competitive mixed inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in Vmax with increase in apparent Km using glycolate as substrate upto 60 microM | Homo sapiens | 4 | ALA5120856 | cell-based format | Scientific Literature | |
| 13. | ALA5123605 | B | Non-competitive mixed inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells assessed as decrease in Vmax with increase in apparent Km using glycolate as substrate upto 60 microM | Mus musculus | 4 | ALA5120856 | cell-based format | Scientific Literature | |
| 14. | ALA5123606 | B | Non-competitive inhibition of human recombinant LDHA assessed as decrease in Vmax with no effect on Km using pyruvate as substrate upto 20 uM | Homo sapiens | 3 | ALA5120856 | single protein format | Scientific Literature | |
| 15. | ALA5123608 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Agxt1 knockdown PH1 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of glycolate relative to control | Mus musculus | 9 | ALA5120856 | cell-based format | Scientific Literature | |
| 16. | ALA5123609 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Agxt1 knockdown PH1 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 48 hrs in the presence of glycolate relative to control | Mus musculus | 8 | ALA5120856 | cell-based format | Scientific Literature | |
| 17. | ALA5123610 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Grhpr-knockdown PH2 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of glycolate relative to control | Mus musculus | 9 | ALA5120856 | cell-based format | Scientific Literature | |
| 18. | ALA5123611 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Grhpr-knockdown PH2 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 48 hrs in the presence of glycolate relative to control | Mus musculus | 9 | ALA5120856 | cell-based format | Scientific Literature | |
| 19. | ALA5123612 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Hoga1-knockdown PH3 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 24 hrs in the presence of hydroxyproline relative to control | Mus musculus | 9 | ALA5120856 | cell-based format | Scientific Literature | |
| 20. | ALA5123613 | B | Inhibition of GO/LDHA in hyperoxaluric-primary hepatocyte isolated from Hoga1-knockdown PH3 C57BL/6 mouse assessed as relative oxalate output at 10 uM measured at 48 hrs in the presence of hydroxyproline relative to control | Mus musculus | 9 | ALA5120856 | cell-based format | Scientific Literature |
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