# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5136790 | B | Inhibition of human full length USP8 overexpressed in baculovirus-infected insect cells using ubiquitin-C-terminal 7-amido-4-methylcoumarin as substrate by fluorescence spectroscopy | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
2. | ALA5136794 | B | Inhibition of N-terminal His6-tagged USP8 (unknown origin) catalytic domain (734 to 1110 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as deubiquitinating activity using ubiquitin-rhodamine -110 as substrate incubated for 30 mins followed by substrate addition measured after 30 mins by ubiquitin-rhodamine-110 based fluorometric assay | Homo sapiens | 20 | cell-based format | Scientific Literature | ||
3. | ALA5136804 | F | Antiproliferative activity against human MCF7 cells assessed as reduction in colony formation at 3 uM incubated for 7 days by crystal violet staining based colony formation assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA5136805 | B | Substrate activity at NQO1 (unknown origin) assessed as reduction in NADPH level measured per umol of NQO1 at 20 uM in presence of NADPH by absorbance based analysis | Homo sapiens | 1 | single protein format | Scientific Literature |