# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5166183 | B | Activation of human 20S proteosome assessed as substrate degradation using Suc-LLVY-AMC/Z-LLE-AMC/Boc-LLR-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis | Homo sapiens | 17 | ALA5154893 | protein complex format | Scientific Literature | |
2. | ALA5166184 | B | Activation of human 20S proteosome assessed as maximum fold change in substrate degradation using Suc-LLVY-AMC/Z-LLE-AMC/Boc-LLR-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis relative to control | Homo sapiens | 9 | ALA5154893 | protein complex format | Scientific Literature | |
3. | ALA5166185 | B | Activation of human 20S proteosome at caspase-like site assessed as substrate degradation using Z-LLE-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis | Homo sapiens | 17 | ALA5154893 | protein complex format | Scientific Literature | |
4. | ALA5166186 | B | Activation of human 20S proteosome at caspase-like site assessed as maximum fold change in substrate degradation using Z-LLE-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis relative to control | Homo sapiens | 6 | ALA5154893 | protein complex format | Scientific Literature | |
5. | ALA5166187 | B | Activation of human 20S proteosome at trypsin-like site assessed as substrate degradation using Boc-LLR-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis | Homo sapiens | 17 | ALA5154893 | protein complex format | Scientific Literature | |
6. | ALA5166188 | B | Activation of human 20S proteosome at trypsin-like site assessed as maximum fold change in substrate degradation using Boc-LLR-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis relative to control | Homo sapiens | 8 | ALA5154893 | protein complex format | Scientific Literature | |
7. | ALA5166189 | B | Activation of human 20S proteosome at chymotrypsin like site assessed as substrate degradation using Suc-LLVY-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis | Homo sapiens | 17 | ALA5154893 | protein complex format | Scientific Literature | |
8. | ALA5166190 | B | Activation of human 20S proteosome at chymotrypsin like site assessed as maximum fold change in substrate degradation using Suc-LLVY-AMC as flurogenic substrate preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis relative to control | Homo sapiens | 7 | ALA5154893 | protein complex format | Scientific Literature | |
9. | ALA5166191 | B | Activation of human 26S proteosome assessed as increase in fold change in proteolytic degradation using SUC-ANW-AMC/Z-LLE-AMC/Boc-LRRAMC as flurogenic substrate at 80 uM preincubated for 15 mins followed by substrate addition and measured after 1 hr by spectrometric analysis relative to control | Homo sapiens | 3 | ALA5154893 | assay format | Scientific Literature | |
10. | ALA5166195 | B | Activation of human 20S-proteosome mediated full length alpha-synuclein degradation by measuring remaining protein at 15 uM incubated for 45 mins by Western blot analysis relative to control | Homo sapiens | 4 | ALA5154893 | assay format | Scientific Literature | |
11. | ALA5166198 | B | Activation of 20S proteasome mediated full length alpha-synuclein A53T mutant degradation expressed in HEK293T cells assessed as residual protein at 15 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA5154893 | cell-based format | Scientific Literature |