Inhibition of Mycobacterium tuberculosis wild type DprE1 expressed in Escherichia coli BL21 DE3 assessed as formation of resorufin using FPR as substrate incubated for 10 mins by Amplex Red dye microplate assay
Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Inhibition of Mycobacterium tuberculosis DprE1 Y314A mutant expressed in Escherichia coli BL21 DE3 assessed as formation of resorufin using FPR as substrate incubated for 10 mins by Amplex Red dye microplate assay
Inhibition of Mycobacterium tuberculosis DprE1 C387A mutant expressed in Escherichia coli BL21 DE3 assessed as formation of resorufin using FPR as substrate incubated for 10 mins by Amplex Red dye microplate assay
Inhibition of Mycobacterium tuberculosis DprE1 C387S mutant expressed in Escherichia coli BL21 DE3 assessed as formation of resorufin using FPR as substrate incubated for 10 mins by Amplex Red dye microplate assay
Inhibition of Mycobacterium tuberculosis DprE1 K418A mutant expressed in Escherichia coli BL21 DE3 assessed as formation of resorufin using FPR as substrate incubated for 10 mins by Amplex Red dye microplate assay
Antimycobacterial activity against rifampin resistant Mycobacterium tuberculosis H37Rv assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against Mycobacterium bovis BCG assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against Mycobacterium bovis BCG overexpressing DprE1 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against Mycobacterium smegmatis mc2155 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against Mycobacterium tuberculosis DprE1 C387S assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against multidrug-resistant Mycobacterium tuberculosis clinical isolate HD1 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against multidrug-resistant Mycobacterium tuberculosis clinical isolate HD2 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against multidrug-resistant Mycobacterium tuberculosis clinical isolate HD3 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against multidrug-resistant Mycobacterium tuberculosis clinical isolate HD4 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against multidrug-resistant Mycobacterium tuberculosis clinical isolate HD5 assessed as inhibition of bacterial growth incubated for 7 days by by microplate alamar blue assay
Antimycobacterial activity against Mycobacterium bovis BCG assessed as reduction in bacterial colony forming units at 20 times MIC concentration measured after 21 days by by time kill assay
Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as reduction in bacterial colony forming units at 20 times MIC concentration measured after 21 days by by time kill assay
Antimycobacterial activity against rifampin resistant Mycobacterium tuberculosis H37Rv assessed as reduction in bacterial colony forming units at 20 times MIC concentration measured after 21 days by by time kill assay
Antimycobacterial activity against Mycobacterium bovis BCG assessed as reduction in bacterial colony forming units at 20 times MIC concentration measured after 7 days by by time kill assay